MicroRNA-21: Expression in oligodendrocytes and correlation with low myelin mRNAs in depression and alcoholism.
MicroRNA-21: Expression in oligodendrocytes and correlation with low myelin mRNAs in depression and alcoholism.
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MicroRNA-21：少突胶质细胞中的表达及其与抑郁症和酗酒中低髓磷脂 mRNA 的相关性。
DOI：
10.1016/j.pnpbp.2017.08.009
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发表时间：
2017-10-03
影响因子：
5.6
通讯作者：
Romero DG
中科院分区：
文献类型：
--
作者：
Miguel-Hidalgo JJ;Hall KO;Bonner H;Roller AM;Syed M;Park CJ;Ball JP;Rothenberg ME;Stockmeier CA;Romero DG
miR-21是一种与癌症、发育和心血管疾病有关的microRNA，在中枢神经系统（CNS）中表达，尤其是在损伤后。然而，miR-21在成年CNS中的细胞表达在小鼠或人类受试者中均未明确确立，而其在精神疾病中的改变尚不清楚。在内源性miR-21启动子（miR-21/LacZ）下表达β-半乳糖苷酶（LacZ）的报告小鼠中表征miR-21表达。在报告小鼠中通过双重免疫荧光检查了miR-21/LacZ与特定神经标志物的脑共定位，而在miR-21敲除小鼠和野生型小鼠中测定了髓鞘碱性蛋白和PDGFRα的免疫染色程度。通过qRT-PCR测量了患有重度抑郁症（MDD）、酒精中毒、MDD+酒精中毒共病（MDA）的人类受试者和非精神病对照受试者的左侧死后眶额皮质（OFC）附近的白色物质（WM）中miR-21和选定的miR-21靶标、miR-21调节剂STAT 3和髓鞘相关蛋白的mRNA水平。MiR-21/LacZ在WM的细胞体和灰质（GM）的有髓鞘部分中高度表达。标记的细胞体被鉴定为少突胶质细胞，而miR-21/LacZ在其他细胞类型中几乎检测不到。MiR-21以及几种髓鞘相关蛋白的mRNA在患有MDD和酒精中毒的受试者的WM中减少。miR-21与髓鞘相关蛋白和星形胶质细胞GFAP的mRNA呈正相关。miR-21在成年少突胶质细胞中的高表达以及miR-21的减少与一些髓鞘蛋白、调节因子STAT 3和少突胶质细胞相关转录因子的mRNA的相关性表明miR-21参与抑郁症和酒精中毒的WM改变。
MiR-21 is a microRNA implicated in cancer, development, and cardiovascular diseases and expressed in the central nervous system (CNS), especially after injury. However, the cellular expression of miR-21 in the adult CNS has not been clearly established either in mice or human subjects, while its alteration in psychiatric disorders is unknown. MiR-21 expression was characterized in reporter mice expressing β–galactosidase (LacZ) under the endogenous miR-21 promoter (miR-21/LacZ). Brain co-localization of miR-21/LacZ with specific neural markers was examined by double immunofluorescence in reporter mice, while extent of immunostaining for myelin basic protein and PDGFRα was determined in miR-21 knockout and wild-type mice. Levels of miR-21, and mRNAs of selected miR-21 targets, miR-21 regulator STAT3 and myelin-related proteins were measured by qRT-PCR in the white matter (WM) adjacent to the left postmortem orbitofrontal cortex (OFC) of human subjects with major depressive disorder (MDD), alcoholism, comorbid MDD plus alcoholism (MDA) and non-psychiatric control subjects. MiR-21/LacZ was highly expressed in cell bodies of WM and myelinated portions of gray matter (GM). Labeled cell bodies were identified as oligodendrocytes, while miR-21/LacZ was barely detectable in other cell types. MiR-21, as well as the mRNAs of several myelin-related proteins, were reduced in the WM of subjects with MDD and alcoholism. MiR-21 positively correlated with mRNA of myelin-related proteins and astrocytic GFAP. High expression of miR-21 in adult oligodendrocytes and the correlation of miR-21 decrease with mRNA of some myelin proteins, regulator STAT3, and oligodendrocyte-related transcription factors suggest an involvement of miR-21 in WM alterations in depression and alcoholism.
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影响因子：
14.2
作者：
Lu, Thomas X.;Rothenberg, Marc E.
通讯作者：
Rothenberg, Marc E.
DOI：
10.4049/jimmunol.1101235
发表时间：
2011-09-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lu TX;Hartner J;Lim EJ;Fabry V;Mingler MK;Cole ET;Orkin SH;Aronow BJ;Rothenberg ME
通讯作者：
Rothenberg ME
影响因子：
4.5
作者：
Cai, XZ;Hagedorn, CH;Cullen, BR
通讯作者：
Cullen, BR
DOI：
10.1111/j.1742-4658.2010.07818.x
发表时间：
2010-10
期刊：
The FEBS journal
影响因子：
--
作者：
Buller B;Liu X;Wang X;Zhang RL;Zhang L;Hozeska-Solgot A;Chopp M;Zhang ZG
通讯作者：
Zhang ZG
DOI：
10.1016/j.biocel.2015.05.021
发表时间：
2015-08-01
影响因子：
4
作者：
Fitzpatrick, John-Mark K.;Anderson, Rebecca C.;McDermott, Kieran W.
通讯作者：
McDermott, Kieran W.