High phylogenetic diversity and abundance pattern of Labyrinthulomycete protists in the coastal waters of the Bohai Sea
High phylogenetic diversity and abundance pattern of Labyrinthulomycete protists in the coastal waters of the Bohai Sea
复制标题
渤海沿岸迷宫菌原生生物的高度系统发育多样性和丰度格局
DOI：
10.1111/1462-2920.14341
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发表时间：
2018-08
影响因子：
5.1
通讯作者：
Wang Guangyi
中科院分区：
文献类型：
--
作者：
Xie Ningdong;Sen Biswarup;Song Zhiquan;Zhao Yue;Chen Zixi;Shi Weijie;Zhang Yongfeng;Zhang Jianle;Johnson Zackary I;Wang Guangyi
长期以来，单细胞唇盘菌类原生生物一直被认为在海洋碳循环中发挥着重要作用。然而，它们的分布和地球化学功能仍然知之甚少。本研究采用流式细胞术和高通量测序技术对渤海沿岸沃茨中的浮游植物进行了大规模的时空丰度和多样性研究。这些原生生物表现出生态位偏好和比浮游细菌更高的生物量，具有更大的系统发育多样性（> 4000 OTU）。它们普遍存在，典型的丰度范围为100-1000个细胞/ml，生物量范围为0.06-574.59 μg/ml。所观察到的时空丰度变化支持目前的“剩余清道夫”的营养模型，并强调这些原生生物作为海洋微生物循环的重要组成部分。近岸沉积物的平均丰度和系统发育多样性高于外海沉积物，表明近岸沉积物在陆源物质分解过程中起主导作用。此外，原生生物属的环境条件的差异关系，以及它们的共生网络表明他们独特的基板偏好和生态位划分。它们的网络拓扑结构显示出群落的弹性和少数几个操作分类单位（OTU）的高连通性，且具有较少的子网和可能的关键种。我们证明了这些原生生物的重要贡献，在沿海沃茨的次级生产和营养循环。作为次级生产者，随着沿海富营养化的加剧，它们的作用将变得更加重要。
The unicellular Labyrinthulomycete protists have long been considered to play a significant role in ocean carbon cycling. However, their distribution and biogeochemical function remain poorly understood. We present a large-scale study of their spatiotemporal abundance and diversity in the coastal waters of Bohai Sea using flow cytometry and high-throughput sequencing. These protists display niche preferences and episodic higher biomass than that of bacterioplankton with much phylogenetic diversity (> 4000 OTUs) ever reported. They were ubiquitous with a typical abundance range of 100-1000 cells ml-1 and biomass range of 0.06-574.59 μg C L-1 . The observed spatiotemporal abundance variations support the current 'left-over scavengers' nutritional model and highlight these protists as a significant component of the marine microbial loop. The higher average abundance and phylogenetic diversity in the nearshore compared with those in the offshore reveal their predominant role in the terrigenous matter decomposition. Furthermore, the differential relationship of the protist genera to environmental conditions together with their co-occurrence network suggests their unique substrate preferences and niche partitioning. With few subnetworks and possible keystone species, their network topology indicates community resilience and high connectance level of few operational taxonomic units (OTUs). We demonstrate the significant contribution of these protists to the secondary production and nutrient cycling in the coastal waters. As secondary producers, their role will become more important with increasingly coastal eutrophication.
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DOI：
10.1007/978-4-431-55130-0_13
发表时间：
2015
期刊：
--
影响因子：
--
作者：
R. Nakai;T. Naganuma
通讯作者：
R. Nakai;T. Naganuma
DOI：
10.1007/978-94-007-5784-4_42
发表时间：
2014
期刊：
--
影响因子：
--
作者：
Z. Finkel
通讯作者：
Z. Finkel
DOI：
10.1007/978-3-319-54304-8
发表时间：
2017
期刊：
--
影响因子：
--
作者：
S. Raghukumar
通讯作者：
S. Raghukumar
DOI：
--
发表时间：
2006-12
期刊：
Polar bioscience
影响因子：
--
作者：
T. Naganuma;Hiroyuki Kimura;R. Karimoto;N. Pimenov
通讯作者：
T. Naganuma;Hiroyuki Kimura;R. Karimoto;N. Pimenov
影响因子：
4.3
作者：
Faust K;Sathirapongsasuti JF;Izard J;Segata N;Gevers D;Raes J;Huttenhower C
通讯作者：
Huttenhower C