Distinct and sex-specific expression of mu opioid receptors in anterior cingulate and somatosensory S1 cortical areas.
Distinct and sex-specific expression of mu opioid receptors in anterior cingulate and somatosensory S1 cortical areas.
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DOI：
10.1097/j.pain.0000000000002751
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发表时间：
2023-04-01
期刊：
影响因子：
7.4
通讯作者：
中科院分区：
文献类型：
--
作者：
补充数字内容在文本中可用。处理疼痛的前扣带回和初级躯体感觉皮质区显示出不同的莫尔表达谱，输出层和性别之间存在显著差异。前扣带皮层（ACC）处理疼痛的情感成分，而初级躯体感觉皮层（S1）参与其感觉辨别成分。据报道，在ACC中注射吗啡具有镇痛作用，并且在该区域中需要内源性阿片类药物来缓解疼痛。Mu阿片受体（MORs）在ACC和S1中都有表达;然而，表达MORs的皮质神经元的身份仍然未知。使用Oprm 1-mCherry小鼠系，我们进行了莫尔+神经元的选择性膜片钳记录，以及经验证的神经元标记物的免疫组织化学，以确定ACC和S1中莫尔+神经元的身份和层状分布。我们发现莫尔+神经元的电生理特征在这两个区域之间存在显著差异，ACC中的类中间神经元放电模式更频繁。而莫尔+生长抑素中间神经元在ACC中更突出，莫尔+兴奋性神经元和莫尔+小清蛋白中间神经元在S1中更突出。我们的研究结果表明，MOR介导的调制ACC和S1输出的差异贡献。我们还发现，女性有一个更大的密度莫尔+神经元相比，男性在这两个领域。总之，我们的结论是，MOR依赖的阿片样物质的信号在皮层显示性二态性，并可能演变为满足边缘系统和感觉皮层区域的疼痛处理电路的独特功能。
Supplemental Digital Content is Available in the Text. Pain-processing anterior cingulate and primary somatosensory cortical areas display distinct MOR expression profiles with prominent differences between output layers and sexes. The anterior cingulate cortex (ACC) processes the affective component of pain, whereas the primary somatosensory cortex (S1) is involved in its sensory-discriminative component. Injection of morphine in the ACC has been reported to be analgesic, and endogenous opioids in this area are required for pain relief. Mu opioid receptors (MORs) are expressed in both ACC and S1; however, the identity of MOR-expressing cortical neurons remains unknown. Using the Oprm1-mCherry mouse line, we performed selective patch clamp recordings of MOR+ neurons, as well as immunohistochemistry with validated neuronal markers, to determine the identity and laminar distribution of MOR+ neurons in ACC and S1. We found that the electrophysiological signatures of MOR+ neurons differ significantly between these 2 areas, with interneuron-like firing patterns more frequent in ACC. While MOR+ somatostatin interneurons are more prominent in ACC, MOR+ excitatory neurons and MOR+ parvalbumin interneurons are more prominent in S1. Our results suggest a differential contribution of MOR-mediated modulation to ACC and S1 outputs. We also found that females had a greater density of MOR+ neurons compared with males in both areas. In summary, we conclude that MOR-dependent opioidergic signaling in the cortex displays sexual dimorphisms and likely evolved to meet the distinct function of pain-processing circuits in limbic and sensory cortical areas.
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影响因子：
5.3
作者：
Loyd, Dayna R.;Murphy, Anne Z.
通讯作者：
Murphy, Anne Z.
影响因子：
13.9
作者：
Corder G;Castro DC;Bruchas MR;Scherrer G
通讯作者：
Scherrer G
影响因子：
13.9
作者：
Fishell G;Rudy B
通讯作者：
Rudy B
影响因子：
25
作者：
Chan KY;Jang MJ;Yoo BB;Greenbaum A;Ravi N;Wu WL;Sánchez-Guardado L;Lois C;Mazmanian SK;Deverman BE;Gradinaru V
通讯作者：
Gradinaru V
影响因子：
2.5
作者：
Coghill, RC;Sang, CN;Iadarola, MJ
通讯作者：
Iadarola, MJ