Citrate-based biphasic scaffolds for the repair of large segmental bone defects.
Citrate-based biphasic scaffolds for the repair of large segmental bone defects.
复制标题
DOI：
10.1002/jbm.a.35228
复制
发表时间：
2015-02
影响因子：
4.9
通讯作者：
Yang, Jian
中科院分区：
文献类型：
--
作者：
Guo, Ying;Tran, Richard T.;Xie, Denghui;Wang, Yuchen;Nguyen, Dianna Y.;Gerhard, Ethan;Guo, Jinshan;Tang, Jiajun;Zhang, Zhongming;Bai, Xiaochun;Yang, Jian
复制天然组织结构的尝试已经导致了专注于改善功能的仿生支架的设计。在这项研究中，仿生柠檬酸盐为基础的聚（柠檬酸辛酯）点击羟基磷灰石（POC-Click-HA）支架的开发，同时复制天然骨组织的组成和建筑特性，同时提供直接的结构支持，大段缺损后植入。制造具有70%内相孔隙率和各种外相孔隙率（5-50%之间）的双相支架，以分别模拟松质骨和皮质骨的双峰分布。双相POC-Click-HA支架显示出高达37.45 ± 3.83 MPa的压缩强度，这可以通过外相孔隙率来控制。还在体内评价了双相支架用于修复兔10 mm长的节段性桡骨缺损，并在植入5、10和15周后与均匀孔隙度的支架以及自体骨移植物进行了比较。结果表明，所有POC-Click-HA支架均具有良好的生物相容性和与宿主骨组织的广泛骨整合。双相支架在植入后的初始阶段显著增强新骨形成，具有较高的骨密度。生物力学和组织形态学分析支持双相支架在早期时间点提供增加的压缩强度、界面骨长入和骨膜重塑的类似结果，但在15周后与所有实验组相当。这些结果证实了双相支架结构在恢复早期阶段恢复骨组织和生理功能的能力，以及基于柠檬酸盐的生物材料在骨科应用中的潜力。
Attempts to replicate native tissue architecture have lead to the design of biomimetic scaffolds focused on improving functionality. In this study, biomimetic citrate-based poly (octanediol citrate) – click hydroxyapatite (POC-Click-HA) scaffolds were developed to simultaneously replicate the compositional and architectural properties of native bone tissue while providing immediate structural support for large segmental defects following implantation. Biphasic scaffolds were fabricated with 70% internal phase porosity and various external phase porosities (between 5–50%) to mimic the bimodal distribution of cancellous and cortical bone, respectively. Biphasic POC-Click-HA scaffolds displayed compressive strengths up to 37.45 ± 3.83 MPa, which could be controlled through the external phase porosity. The biphasic scaffolds were also evaluated in vivo for the repair of 10-mm long segmental radial defects in rabbits and compared to scaffolds of uniform porosity as well as autologous bone grafts after 5, 10, and 15 weeks of implantation. The results showed that all POC-Click-HA scaffolds exhibited good biocompatibility and extensive osteointegration with host bone tissue. Biphasic scaffolds significantly enhanced new bone formation with higher bone densities in the initial stages after implantation. Biomechanical and histomorphometric analysis supported a similar outcome with biphasic scaffolds providing increased compression strength, interfacial bone ingrowth, and periosteal remodeling in early time points, but were comparable to all experimental groups after 15 weeks. These results confirm the ability of biphasic scaffold architectures to restore bone tissue and physiological functions in the early stages of recovery, and the potential of citrate-based biomaterials in orthopedic applications.
登录
查看更多内容
DOI：
10.1007/s10856-007-3028-3
发表时间：
2007-09-01
影响因子：
3.7
作者：
Heilmann, F.;Standard, O. C.;Hoffman, M.
通讯作者：
Hoffman, M.
影响因子：
3.4
作者：
Jiao Y;Gyawali D;Stark JM;Akcora P;Nair P;Tran RT;Yang J
通讯作者：
Yang J
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
DOI：
10.2174/1876525401204010027
发表时间：
2012-01-01
期刊：
The Open bone journal
影响因子：
--
作者：
Costello, Leslie C;Franklin, Renty B;Chellaiah, Meena
通讯作者：
Chellaiah, Meena
骨组织工程多层仿生支架的制备及表征
影响因子：
2.9
作者：
Kong, Lijun;Ao, Qiang;Zhang, Xiufang
通讯作者：
Zhang, Xiufang