Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis.
Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis.
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DOI：
10.1186/s12864-020-07309-z
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发表时间：
2021-01-06
期刊：
影响因子：
4.4
通讯作者：
Rikihisa Y
中科院分区：
文献类型：
--
作者：
Lin M;Xiong Q;Chung M;Daugherty SC;Nagaraj S;Sengamalay N;Ott S;Godinez A;Tallon LJ;Sadzewicz L;Fraser C;Dunning Hotopp JC;Rikihisa Y
埃利希体属由蜱传播的强制性细胞内细菌组成，可引起具有医学和农业重要性的致命疾病。埃利希体HF 是从日本的卵形硬蜱中分离出来的[也称为卵形 I. ovatus 埃利希体 (IOE) 病原体]，可在实验室小鼠中引起急性致命感染，类似于由恰菲埃利希体引起的急性致命人类单核细胞埃利希体病。由于没有小型实验动物模型来研究致命的人类埃利希体病，埃利希体属。 HF 提供了所需的疾病模型。然而，无法培养埃里希体。心衰和基因组信息的缺乏一直是推进这种动物模型的障碍。此外，埃利希体 sp。 HF 在文献中有多个名称，因为它缺乏分类学上认可的名称。我们稳定培养了埃利希体。 HF在犬组织细胞白血病DH82细胞中取自HF株感染的小鼠，并测定了其完整的基因组序列。埃利希体HF 具有 1,148,904 bp 的单链双链环状染色体，编码 866 个蛋白质，其代谢潜力与查菲埃里希体相似。埃利希体HF 编码查菲埃里希体中发现的所有毒力因子的同源物，包括 P28/OMP-1 家族外膜蛋白的 23 个旁系同源物、IV 型分泌系统装置和效应蛋白、双组分系统、锚蛋白重复蛋白和串联重复蛋白。埃利希体HF 是埃利希体属中的一个新物种，通过使用平均核苷酸同一性、数字 DNA-DNA 杂交和核心基因组比对序列同一性与六个代表性埃利希体物种、亚种和菌株的全基因组比较证明了 HF 是埃利希体属中的新物种。埃里希体属的基因组。 HF 编码查菲埃利希体中发现的所有已知毒力因子，证实其是研究致命性人类埃利希体病的模型埃利希体。埃里希体 sp. 之间的比较。 HF 和查菲埃里希菌将能够鉴定与宿主特异性、疾病严重程度和宿主炎症反应相关的体内毒力因子。我们建议将其命名为 Ehrlichia sp。 HF 为日本埃里希体 (Ehrlichia japonica sp.)。十一月（HF 型菌株），表示该细菌最初分离的地理区域。在线版本包含可在 10.1186/s12864-020-07309-z 获取的补充材料。
The genus Ehrlichia consists of tick-borne obligatory intracellular bacteria that can cause deadly diseases of medical and agricultural importance. Ehrlichia sp. HF, isolated from Ixodes ovatus ticks in Japan [also referred to as I. ovatus Ehrlichia (IOE) agent], causes acute fatal infection in laboratory mice that resembles acute fatal human monocytic ehrlichiosis caused by Ehrlichia chaffeensis. As there is no small laboratory animal model to study fatal human ehrlichiosis, Ehrlichia sp. HF provides a needed disease model. However, the inability to culture Ehrlichia sp. HF and the lack of genomic information have been a barrier to advance this animal model. In addition, Ehrlichia sp. HF has several designations in the literature as it lacks a taxonomically recognized name. We stably cultured Ehrlichia sp. HF in canine histiocytic leukemia DH82 cells from the HF strain-infected mice, and determined its complete genome sequence. Ehrlichia sp. HF has a single double-stranded circular chromosome of 1,148,904 bp, which encodes 866 proteins with a similar metabolic potential as E. chaffeensis. Ehrlichia sp. HF encodes homologs of all virulence factors identified in E. chaffeensis, including 23 paralogs of P28/OMP-1 family outer membrane proteins, type IV secretion system apparatus and effector proteins, two-component systems, ankyrin-repeat proteins, and tandem repeat proteins. Ehrlichia sp. HF is a novel species in the genus Ehrlichia, as demonstrated through whole genome comparisons with six representative Ehrlichia species, subspecies, and strains, using average nucleotide identity, digital DNA-DNA hybridization, and core genome alignment sequence identity. The genome of Ehrlichia sp. HF encodes all known virulence factors found in E. chaffeensis, substantiating it as a model Ehrlichia species to study fatal human ehrlichiosis. Comparisons between Ehrlichia sp. HF and E. chaffeensis will enable identification of in vivo virulence factors that are related to host specificity, disease severity, and host inflammatory responses. We propose to name Ehrlichia sp. HF as Ehrlichia japonica sp. nov. (type strain HF), to denote the geographic region where this bacterium was initially isolated. The online version contains supplementary material available at 10.1186/s12864-020-07309-z.
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影响因子：
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作者：
Auch AF;von Jan M;Klenk HP;Göker M
通讯作者：
Göker M
影响因子：
9.4
作者：
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通讯作者：
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影响因子：
3.4
作者：
Cheng, Zhihui;Kumagai, Yumi;Rikihisa, Yasuko
通讯作者：
Rikihisa, Yasuko
影响因子：
4.4
作者：
Bitsaktsis, C;Huntington, J;Winslow, G
通讯作者：
Winslow, G
DOI：
10.1099/ijsem.0.000895
发表时间：
2016-03-01
影响因子：
2.8
作者：
Cabezas-Cruz, Alejandro;Zweygarth, Erich;de la Fuente, Jose
通讯作者：
de la Fuente, Jose