Targeted integration of genes in Xenopus tropicalis

Targeted integration of genes in Xenopus tropicalis
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热带爪蟾基因的靶向整合

DOI:
10.1002/dvg.23006
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发表时间:
2017-01
期刊:
影响因子:
1.5
通讯作者:
Chen Yonglong
Chen Yonglong
中科院分区:
生物学4区
文献类型:
--
作者:
Shi Zhaoying;Tian D;an;Xin Huhu;Lian Jingru;Guo Xiaogang;Chen Yonglong

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随着在真正的二倍体青蛙热带爪蟾中成功建立靶向基因破坏和整合方法,这种优秀的脊椎动物遗传模型现在正在为模拟人类疾病做出独特的贡献。在这里,我们总结了我们在热带爪蟾中建立同源重组介导的靶向整合的努力,通过同源非依赖策略的靶向整合的有用性和局限性,以及未来如何进一步提高热带爪蟾中靶向基因整合的方向。
With the successful establishment of both targeted gene disruption and integration methods in the true diploid frog Xenopus tropicalis, this excellent vertebrate genetic model now is making a unique contribution to modelling human diseases. Here, we summarize our efforts on establishing homologous recombination‐mediated targeted integration in Xenopus tropicalis, the usefulness, and limitation of targeted integration via the homology‐independent strategy, and future directions on how to further improve targeted gene integration in Xenopus tropicalis.
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