Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals.
Septin filaments exhibit a dynamic, paired organization that is conserved from yeast to mammals.
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DOI：
10.1083/jcb.201012143
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发表时间：
2011-06-13
期刊：
影响因子：
--
通讯作者：
Gladfelter AS
中科院分区：
文献类型：
--
作者：
DeMay BS;Bai X;Howard L;Occhipinti P;Meseroll RA;Spiliotis ET;Oldenbourg R;Gladfelter AS
偏振荧光显微镜显示，不同物种的septins聚集成类似的高阶结构，由动态的成对细丝组成。septin是保守的gtp结合蛋白，对细胞质分裂、膜区隔化和胞吐作用很重要。然而，尚不清楚septin是如何在细胞的高阶结构中排列的。为了确定活细胞中septin的组织，我们开发了一种偏振荧光显微镜系统，以高空间和时间分辨率监测GFP偶极矩的方向。当GFP与septin融合时，GFP偶极子的排列反映了潜在的septin组织。我们在丝状真菌，出芽酵母和哺乳动物上皮细胞系中证明了septin蛋白以相同的高度有序的方式组织。荧光各向异性测量表明，septin丝在活细胞内组织成对，正如在体外观察到的那样。对成对形成的额外支持来自电子显微镜对细胞皮层成对细丝的观察。此外，我们发现高度有序的septin结构交换亚基并迅速重排。我们得出结论，septin在体内组装成动态的，成对的细丝，并且这种组织从酵母到哺乳动物都是保守的。
Polarized fluorescence microscopy reveals that septins across diverse species assemble into similar higher-order structures consisting of dynamic, paired filaments. The septins are conserved, GTP-binding proteins important for cytokinesis, membrane compartmentalization, and exocytosis. However, it is unknown how septins are arranged within higher-order structures in cells. To determine the organization of septins in live cells, we developed a polarized fluorescence microscopy system to monitor the orientation of GFP dipole moments with high spatial and temporal resolution. When GFP was fused to septins, the arrangement of GFP dipoles reflected the underlying septin organization. We demonstrated in a filamentous fungus, a budding yeast, and a mammalian epithelial cell line that septin proteins were organized in an identical highly ordered fashion. Fluorescence anisotropy measurements indicated that septin filaments organized into pairs within live cells, just as has been observed in vitro. Additional support for the formation of pairs came from the observation of paired filaments at the cortex of cells using electron microscopy. Furthermore, we found that highly ordered septin structures exchanged subunits and rapidly rearranged. We conclude that septins assemble into dynamic, paired filaments in vivo and that this organization is conserved from yeast to mammals.
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影响因子：
2.9
作者：
DeMay, Bradley S.;Meseroll, Rebecca A.;Gladfelter, Amy S.
通讯作者：
Gladfelter, Amy S.
影响因子：
3.5
作者：
Peterson EA;Petty EM
通讯作者：
Petty EM
影响因子：
3.4
作者：
Pan F;Malmberg RL;Momany M
通讯作者：
Momany M
纯化的酵母菌的聚合：Septin功能可能不需要有组织的丝阵列。
影响因子：
7.8
作者：
Frazier, J A;Wong, M L;Longtine, M S;Pringle, J R;Mann, M;Mitchison, T J;Field, C
通讯作者：
Field, C
DOI：
10.1083/jcb.69.3.717
发表时间：
1976-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Byers B;Goetsch L
通讯作者：
Goetsch L