Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited.
Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited.
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DOI：
10.1084/jem.181.1.257
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发表时间：
1995-01-01
影响因子：
15.3
通讯作者：
Horwitz, Marcus A.
中科院分区：
文献类型：
--
作者：
Clemens, Daniel L.;Horwitz, Marcus A.
我们用冰冻切片免疫金技术研究了结核分枝杆菌吞噬体的组成。我们已经使用定量免疫金染色来确定几种已知的内体-溶酶体途径标记物在人单核细胞摄入M。结核杆菌、嗜肺军团菌或聚苯乙烯珠。与其它吞噬颗粒相比，M.结核病吞噬体表现出对主要组织相容性复合体（MHC）I类分子的延迟清除，对MHC II类分子和内体标记转铁蛋白受体的相对强的染色，以及对溶酶体膜糖蛋白、CD 63、LAMP-1和LAMP-2以及溶酶体酸性蛋白酶、组织蛋白酶D的相对弱的染色。与M.肺结核、L.嗜肺菌吞噬体迅速清除MHC I类分子，并排除所有研究的内体-溶酶体标志物。与此同时，两个活M。结核和L.嗜肺菌吞噬体、含有聚苯乙烯珠粒或热灭活M.结核杆菌对溶酶体膜糖蛋白和组织蛋白酶D染色较强。这些结果表明：（a）M.结核病延缓其吞噬体沿着内体-溶酶体途径的成熟，并驻留在具有内体而不是溶酶体特征的区室中;和（B）吞噬体内途径，即，抑制吞噬体-溶酶体融合的几种细胞内寄生虫遵循的途径是异质的。
We have used the cryosection immunogold technique to study the composition of the Mycobacterium tuberculosis phagosome. We have used quantitative immunogold staining to determine the distribution of several known markers of the endosomal-lysosomal pathway in human monocytes after ingestion of either M. tuberculosis, Legionella pneumophila, or polystyrene beads. Compared with the other phagocytic particles studied, the M. tuberculosis phagosome exhibits delayed clearance of major histocompatibility complex (MHC) class I molecules, relatively intense staining for MHC class II molecules and the endosomal marker transferrin receptor, and relatively weak staining for the lysosomal membrane glycoproteins, CD63, LAMP-1, and LAMP-2 and the lysosomal acid protease, cathepsin D. In contrast to M. tuberculosis, the L. pneumophila phagosome rapidly clears MHC class I molecules and excludes all endosomal-lysosomal markers studied. In contrast to both live M. tuberculosis and L. pneumophila phagosomes, phagosomes containing either polystyrene beads or heat-killed M. tuberculosis stain intensely for lysosomal membrane glycoproteins and cathepsin D. These findings suggest that (a) M. tuberculosis retards the maturation of its phagosome along the endosomal-lysosomal pathway and resides in a compartment with endosomal, as opposed to lysosomal, characteristics; and (b) the intraphagosomal pathway, i.e., the pathway followed by several intracellular parasites that inhibit phagosome-lysosome fusion, is heterogeneous.
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吞噬作用期间的膜分选：选择性排除主要的组织相容性复合物分子，但在常规和盘绕吞噬作用期间不补充受体CR3。
影响因子：
15.3
作者：
CLEMENS, DL;HORWITZ, MA
通讯作者：
HORWITZ, MA
细胞毒性T淋巴细胞颗粒是分泌的溶酶体，含有穿孔蛋白和颗粒酶。
DOI：
10.1084/jem.173.5.1099
发表时间：
1991-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Peters PJ;Borst J;Oorschot V;Fukuda M;Krähenbühl O;Tschopp J;Slot JW;Geuze HJ
通讯作者：
Geuze HJ
DOI：
10.1073/pnas.87.14.5553
发表时间：
1990-07-01
影响因子：
11.1
作者：
HARDING, CV;UNANUE, ER;GEUZE, HJ
通讯作者：
GEUZE, HJ
影响因子：
7
作者：
HORWITZ, MA
通讯作者：
HORWITZ, MA
影响因子：
7.8
作者：
RABINOWITZ, S;HORSTMANN, H;GRIFFITHS, G
通讯作者：
GRIFFITHS, G