Algicidal Activity of Novel Marine Bacterium Paracoccus sp. Strain Y42 against a Harmful Algal-Bloom-Causing Dinoflagellate, Prorocentrum donghaiense
Algicidal Activity of Novel Marine Bacterium Paracoccus sp. Strain Y42 against a Harmful Algal-Bloom-Causing Dinoflagellate, Prorocentrum donghaiense
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新型海洋细菌副球菌的杀藻活性。
DOI：
10.1128/aem.01015-18
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发表时间：
2018-07
影响因子：
4.4
通讯作者：
Hong Xu
中科院分区：
文献类型：
--
作者：
Fuxing Zhang;Qian Ye;Qiuliang Chen;Ke Yang;Danyang Zhang;Zhangran Chen;Shasha Lu;Xueping Shao;Yongxiang Fan;Luming Yao;Lina Ke;Tianling Zheng;Hong Xu
东海甲藻是最常见的甲藻之一，其赤潮的发生会造成严重的生态污染，并对人类和其他动物的健康造成危害。筛选对东海原甲藻有较高杀藻活性的细菌，研究其杀藻过程和特性，有助于了解其杀藻效果，为防治赤潮、减少其对环境的危害提供理论依据。本文报道了副球菌对东海原甲藻的溶藻活性及其特性。副球菌在不同环境（包括不同温度、pH和光照条件）下的溶藻活性稳定性表明其在东海副球菌水华控制中具有潜在的应用价值。摘要东海原甲藻水华在长江口及邻近海域频繁发生。在过去20年里，这些水华破坏了海洋生态系统，造成了巨大的经济损失。因此，必须开发高效、低成本、生态友好的方法来控制东海原甲藻水华。本研究将一株菌株Y42鉴定为副球菌属（Paracoccus sp.），并将其用于裂解东海原甲藻。Y42菌株培养上清对东海原甲藻有一定的溶藻作用，其溶藻活性在不同温度、光照时间和较宽的pH值范围内稳定。除东海原甲藻外，Y42对微小亚历山大藻、锥状斯氏藻和中肋骨条藻也表现出较高的杀藻活性，表明Y42主要以甲藻为靶标。为了阐明Y42的杀藻效果，我们研究了Y42上清液对东海原甲藻的溶藻作用，并测定了Y42上清液对东海原甲藻叶绿素a含量、光合活性和丙二醛含量的影响。扫描电子显微镜和透射电子显微镜分析表明，Y42上清液破坏膜的完整性，并导致藻细胞在巨细胞区的断裂。光合色素损失、光合效率和电子传递速率均显著下降，表明Y42培养上清液对东海原甲藻的光合系统造成了损害。丙二醛的过量产生表明，Y42上清液引起脂质过氧化和氧化损伤的细胞膜系统，最终导致死亡。本研究结果揭示了Y42去除东海原甲藻水华中藻细胞的潜力。重要性东海甲藻（P. donghaiense）是最常见的甲藻种类之一，其形成的有害藻华经常造成严重的生态污染，并对人类和其他动物的健康构成危害。筛选对东海原甲藻有较高杀藻活性的细菌，研究其杀藻过程和特性，有助于了解其杀藻效果，为防治赤潮、减少其对环境的危害提供理论依据。本文报道了副球菌对东海原甲藻的溶藻活性及其特性。副球菌在不同环境（包括不同温度、pH和光照条件）下的溶藻活性稳定性表明其在东海副球菌水华控制中具有潜在的应用价值。
P. donghaiense is one of the most common dinoflagellate species that form harmful algal blooms, which frequently cause serious ecological pollution and pose health hazards to humans and other animals. Screening for bacteria with high algicidal activity against P. donghaiense and studying their algicidal processes and characteristics will contribute to an understanding of their algicidal effects and provide a theoretical basis for preventing algal blooms and reducing their harm to the environment. This study reports the algicidal activity and characteristics of Paracoccus against P. donghaiense. The stability of the algicidal activity of Paracoccus in different environments (including different temperature, pH, and sunlight conditions) indicates its potential for use in the control of P. donghaiense blooms. ABSTRACT Prorocentrum donghaiense blooms occur frequently in the Yangtze River estuary and the adjacent East China Sea. These blooms have damaged marine ecosystems and caused enormous economic losses over the past 2 decades. Thus, highly efficient, low-cost, ecofriendly approaches must be developed to control P. donghaiense blooms. In this study, a bacterial strain (strain Y42) was identified as Paracoccus sp. and was used to lyse P. donghaiense. The supernatant of the strain Y42 culture was able to lyse P. donghaiense, and the algicidal activity of this Y42 supernatant was stable with different temperatures and durations of light exposure and over a wide pH range. In addition to P. donghaiense, Y42 showed high algicidal activity against Alexandrium minutum, Scrippsiella trochoidea, and Skeletonema costatum, suggesting that it targets primarily Pyrrophyta. To clarify the algicidal effects of Y42, we assessed algal lysis and determined the chlorophyll a contents, photosynthetic activity, and malondialdehyde contents of P. donghaiense after exposure to the Y42 supernatant. Scanning electron microscopy and transmission electron microscopy analyses showed that the Y42 supernatant disrupted membrane integrity and caused algal cell breakage at the megacytic zone. Photosynthetic pigment loss and significant declines in both photosynthetic efficiency and the electron transport rate indicated that the Y42 supernatant damaged the photosynthetic system of P. donghaiense. Malondialdehyde overproduction indicated that the Y42 supernatant caused lipid peroxidation and oxidative damage to membrane systems in the algal cell, ultimately leading to death. The findings of this study reveal the potential of Y42 to remove algal cells from P. donghaiense blooms. IMPORTANCE P. donghaiense is one of the most common dinoflagellate species that form harmful algal blooms, which frequently cause serious ecological pollution and pose health hazards to humans and other animals. Screening for bacteria with high algicidal activity against P. donghaiense and studying their algicidal processes and characteristics will contribute to an understanding of their algicidal effects and provide a theoretical basis for preventing algal blooms and reducing their harm to the environment. This study reports the algicidal activity and characteristics of Paracoccus against P. donghaiense. The stability of the algicidal activity of Paracoccus in different environments (including different temperature, pH, and sunlight conditions) indicates its potential for use in the control of P. donghaiense blooms.
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影响因子：
56.9
作者：
Teeling, Hanno;Fuchs, Bernhard M.;Amann, Rudolf
通讯作者：
Amann, Rudolf
DOI：
10.1007/s11270-014-2120-9
发表时间：
2014-10
期刊：
Water, Air, & Soil Pollution
影响因子：
--
作者：
Chunli Liao;Xiaobo Liu
通讯作者：
Chunli Liao;Xiaobo Liu
杀藻细菌诱导后，有害藻华中的细胞死亡导致塔玛亚历山大藻物种死亡
影响因子：
5
作者：
Zhang Huajun;Lv Jinglin;Peng Yun;Zhang Su;An Xinli;Xu Hong;Zhang Jun;Tian Yun;Zheng Wei;Zheng Tianling
通讯作者：
Zheng Tianling
DOI：
--
发表时间：
2015
期刊：
--
影响因子：
--
作者：
D. Kidwell
通讯作者：
D. Kidwell
DOI：
10.1128/genomea.00571-17
发表时间：
2017-06-29
期刊：
Genome announcements
影响因子：
--
作者：
El Houmami N;Schrenzel J;Yagupsky P;Robert C;Ceroni D;Raoult D;Fournier PE
通讯作者：
Fournier PE