A verion‐like particle (VLP) released from an epstein‐barr virus‐producing cell sub‐line of P3HR‐1: Identification of a specific cellular DNA contained within VLPs
A verion‐like particle (VLP) released from an epstein‐barr virus‐producing cell sub‐line of P3HR‐1: Identification of a specific cellular DNA contained within VLPs
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从产生 epstein-barr 病毒的 P3HR-1 细胞亚系中释放的版本样颗粒 (VLP)：鉴定 VLP 中包含的特定细胞 DNA
DOI：
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发表时间：
1986
影响因子：
6.4
通讯作者：
T. Osato
中科院分区：
文献类型：
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作者：
S. Yano;I. Miyoshi;Y. Ohtsuki;S. Koizumi;T. Osato
从P3 HR-1细胞的培养液中分离出视觉样颗粒（VLP）。由于VLP密度较大，可通过速率-速度沉降法将其与有包膜的爱泼斯坦-巴尔病毒（EBV）分离。VLP包含电子致密的核苷酸物质和周围的圆形或椭圆形包膜，直径范围为100至150 nm。SDS-聚丙烯酰胺凝胶电泳显示VLP的多肽组成与包膜EBV非常相似。VLP含有沉降系数为48 S的合理特异性细胞DNA（VLP DNA），其通过用BamHI、Sal和EcoRI消化分别被切割成5、8和至少30个特异性DNA片段。印迹杂交数据表明VLP DNA的有限异质性基因组组织和人脐带淋巴细胞中存在VLP DNA相关分子。
A vision‐like particle (VLP) was isolated from the culture fluid of P3HR‐1 cells. VLPs were separable from the enveloped Epstein‐Barr virus (EBV) through rate‐velocity sedimentation by virtue of their greater density. VLPs comprised electron‐dense nucleotide material and the surrounding round or oval envelope which ranged from 100 to 150 nm in diameter. The polypeptide constitution of VLPs was very similar to that of enveloped EBV by SDS‐polyacrylamide gel electrophoresis. VLPs contained reasonably specific cellular DNA (VLP DNA) with a sedimentation coefficient of 48S, which was cleaved into 5, 8, and at least 30 specific DNA fragments by digestion with BamHI, Sal, and EcoRI, respectively. Blot hybridization data suggested a limited heterogeneous genomic organization of VLP DNA and the presence of a VLP DNA‐related molecule(s) in human cord lymphocytes.
P3HR-1 的 epstein-Barr 病毒 (EBV) 分析：分离出在人脐带淋巴细胞中具有 EBNA 诱导能力且在 Raji 细胞中不具有 EA 诱导能力的 EBV。
影响因子：
3.7
作者：
Yano,S;Tanaka,A;Takada,K;Fujiwara,S;Osato,T;Nonoyama,M
通讯作者：
Nonoyama,M
将 DNA、RNA 和蛋白质电泳转移到重氮苄氧基甲基 (DBM) 纸上。
影响因子：
14.9
作者：
Stellwag,EJ;Dahlberg,AE
通讯作者：
Dahlberg,AE