A doublecortin containing microtubule-associated protein is implicated in mechanotransduction in Drosophila sensory cilia.
A doublecortin containing microtubule-associated protein is implicated in mechanotransduction in Drosophila sensory cilia.
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DOI：
10.1038/ncomms1007
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发表时间：
2010-04-12
影响因子：
16.6
通讯作者：
Howard, J.
中科院分区：
文献类型：
--
作者：
Bechstedt, S.;Albert, J. T.;Kreil, D. P.;Mueller-Reichert, T.;Goepfert, M. C.;Howard, J.
机械感受器是将机械刺激转化为电信号并介导声音、触觉和加速度感知的感觉细胞。纤毛机械感受器具有精细的微管细胞骨架，其有利于将外力耦合到转导装置。在筛选果蝇campaniform mechanoreceptor中优先表达的基因时，我们鉴定了DCX-EMAP，它是EMAP家族（棘皮动物微管相关蛋白）的一个独特成员，包含两个doublecortin结构域。DCX-EMAP定位于钟状感受器中的管状体和约翰斯顿器官（果蝇的听觉器官）中的弦音机械感受器中的纤毛扩张。在DCX-EMAP基因中携带piggyBac插入的成年果蝇是不协调的和耳聋的，并且显示机械感觉转导和放大的损失。电子显微镜的突变体的感受器揭示了电子致密材料的损失内的微管细胞骨架中的管状体和纤毛扩张。我们的研究结果建立了果蝇mechanosensory的候选基因目录，并表明一个候选人，DCX-EMAP，可能是所需的mechanosensory转导和放大。机械感受器对于检测外部刺激是重要的。在这项研究中，一个假定的受体确定在果蝇中被证明是潜在的重要听力和放大mechanotransduction。
Mechanoreceptors are sensory cells that transduce mechanical stimuli into electrical signals and mediate the perception of sound, touch and acceleration. Ciliated mechanoreceptors possess an elaborate microtubule cytoskeleton that facilitates the coupling of external forces to the transduction apparatus. In a screen for genes preferentially expressed in Drosophila campaniform mechanoreceptors, we identified DCX-EMAP, a unique member of the EMAP family (echinoderm–microtubule-associated proteins) that contains two doublecortin domains. DCX-EMAP localizes to the tubular body in campaniform receptors and to the ciliary dilation in chordotonal mechanoreceptors in Johnston's organ, the fly's auditory organ. Adult flies carrying a piggyBac insertion in the DCX-EMAP gene are uncoordinated and deaf and display loss of mechanosensory transduction and amplification. Electron microscopy of mutant sensilla reveals loss of electron-dense materials within the microtubule cytoskeleton in the tubular body and ciliary dilation. Our results establish a catalogue of candidate genes for Drosophila mechanosensation and show that one candidate, DCX-EMAP, is likely to be required for mechanosensory transduction and amplification. Mechanoreceptors are important for detecting external stimuli. In this study a putative receptor identified in Drosophila is shown to be potentially important for hearing and the amplification of mechanotransduction.
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DOI：
10.1007/bf00306874
发表时间：
1972-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
CHUWANG, I;AXTELL, RC
通讯作者：
AXTELL, RC
影响因子：
4.8
作者：
Eichenmüller, B;Everley, P;Suprenant, KA
通讯作者：
Suprenant, KA
DOI：
10.1016/j.cub.2008.11.020
发表时间：
2008-12-23
期刊：
Current biology : CB
影响因子：
--
作者：
Lee E;Sivan-Loukianova E;Eberl DF;Kernan MJ
通讯作者：
Kernan MJ
影响因子：
56.9
作者：
Chan, WP;Prete, F;Dickinson, MH
通讯作者：
Dickinson, MH
影响因子：
64.5
作者：
Avidor-Reiss, T;Maer, AM;Zuker, CS
通讯作者：
Zuker, CS