An IFT-A protein is required to delimit functionally distinct zones in mechanosensory cilia.
An IFT-A protein is required to delimit functionally distinct zones in mechanosensory cilia.
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DOI：
10.1016/j.cub.2008.11.020
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发表时间：
2008-12-23
期刊：
影响因子：
--
通讯作者：
Kernan MJ
中科院分区：
文献类型：
--
作者：
Lee E;Sivan-Loukianova E;Eberl DF;Kernan MJ
大多数真核生物纤毛和鞭毛的组装需要保守的鞭毛内运输（IFT）颗粒蛋白和IFT相关马达。从纤毛尖端到基部，动力蛋白驱动的逆行IFT通常需要IFT- a亚复合物中的蛋白质。我们描述了新的结构和功能作用的IFT-A蛋白在chordotonal器官，昆虫机械感觉器官与纤毛，既感觉和运动。果蝇的机械受体电位A （rempA）位点编码IFT-A成分IFT140。在rempA突变体中，Chordotonal纤毛缩短，并且在突变体纤毛中积累了IFT- b蛋白，这与逆行IFT的缺陷一致。一个功能性REMPA-YFP融合蛋白集中在纤毛扩张（CD）部位，这是一个高度结构的轴突包涵体，迄今为止未知的成分和功能。在rempA突变体中不存在CD，而在缺乏另一种IFT-A蛋白IFT122时，rempA - yfp无法检测到。在缺乏IFT动力蛋白马达的突变体中，CD紊乱，REMPA-YFP定位错误。TRPV离子通道是产生感觉电位和调节纤毛运动所必需的，通常位于纤毛的近端，在dynein突变体中，该通道扩展到纤毛的远端，而在rempA突变体中则无法检测到。IFT-A蛋白位于纤毛扩张处并为纤毛扩张所必需，纤毛扩张将弦状纤毛分离为功能不同的区域。TRPV通道的稳定表达需要IFT140，这也表明IFT-A蛋白可能介导一些膜蛋白的前毛转运。
Conserved intraflagellar transport (IFT) particle proteins and IFT-associated motors are needed to assemble most eukaryotic cilia and flagella. Proteins in an IFT-A subcomplex are generally required for dynein-driven retrograde IFT, from the ciliary tip to the base. We describe novel structural and functional roles for IFT-A proteins in chordotonal organs, insect mechanosensory organs with cilia that are both sensory and motile. The reduced mechanoreceptor potential A (rempA) locus of Drosophila encodes the IFT-A component IFT140. Chordotonal cilia are shortened in rempA mutants and an IFT-B protein accumulates in the mutant cilia, consistent with a defect in retrograde IFT. A functional REMPA-YFP fusion protein concentrates at the site of the ciliary dilation (CD), a highly structured axonemal inclusion of hitherto unknown composition and function. The CD is absent in rempA mutants, and REMPA-YFP is undetectable in the absence of another IFT-A protein, IFT122. In a mutant lacking the IFT dynein motor, the CD is disorganized and REMPA-YFP is mislocalized. A TRPV ion channel, required to generate sensory potentials and regulate ciliary motility, is normally localized in the cilia, proximal to the CD. This channel spreads into the distal part of the cilia in dynein mutants, and is undetectable in rempA mutants. IFT-A proteins are located at and required by the ciliary dilation, which separates chordotonal cilia into functionally distinct zones. A requirement for IFT140 in stable TRPV channel expression also suggests that IFT-A proteins may mediate preciliary transport of some membrane proteins.
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影响因子：
4.6
作者：
Bae, Young-Kyung;Qin, Hongmin;Barr, Maureen M.
通讯作者：
Barr, Maureen M.
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL
影响因子：
4
作者：
Jékely, G;Arendt, D
通讯作者：
Arendt, D
影响因子：
5.3
作者：
Gong, ZF;Son, WS;Kim, CS
通讯作者：
Kim, CS
影响因子：
64.8
作者：
Kim, J;Chung, YD;Kim, C
通讯作者：
Kim, C