Chlamydial development is blocked in host cells transfected with Chlamydophila caviae incA.
Chlamydial development is blocked in host cells transfected with Chlamydophila caviae incA.
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DOI：
10.1186/1471-2180-4-24
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发表时间：
2004-07-01
期刊：
影响因子：
4.2
通讯作者：
Rockey DD
中科院分区：
文献类型：
--
作者：
Alzhanov D;Barnes J;Hruby DE;Rockey DD
衣原体产生一组蛋白质，称为Inc蛋白，其定位于包涵体膜并暴露于宿主细胞胞质溶胶。关于Inc蛋白与真核细胞的相互作用的信息很少。为了检查这些相互作用，使用牛痘病毒载体和基于哺乳动物质粒的系统在哺乳动物细胞中表达inc基因。用表达嗜衣原体印加的质粒转染的细胞不能被C.开玩笑。表达C.印加也减少了沙眼衣原体的包涵体形成，但没有达到C.开玩笑。沙眼衣原体印加不能阻断两种衣原体的发育。trachomatis或C.开玩笑。用缺失突变证明编码蛋白质氨基或羧基末端区域的质粒，以及印加内单个氨基酸（丝氨酸17）的改变不能阻断C。败血症感染免疫印迹分析表明，在牛痘病毒系统中截短的印加的丝氨酸17。钙调素印加是磷酸化的靶点。这些实验提供了深入了解与宿主细胞的蛋白质的相互作用，并引入了一个模型系统，这些相互作用可以进一步探讨。
Chlamydiae produce a set of proteins, termed Inc proteins, that are localized to the inclusion membrane and exposed to the host cell cytosol. Little information exists regarding the interaction of Inc proteins with the eukaryotic cell. To examine these interactions, Vaccinia virus vectors and mammalian plasmid-based systems were used to express inc genes in mammalian cells. Cells transfected with plasmids expressing Chlamydophila caviae incA were not productively infected by C. caviae. Expression of C. caviae incA also reduced inclusion formation by Chlamydia trachomatis, but not to the degree seen for C. caviae. Chlamydia trachomatis incA did not block development of either C. trachomatis or C. caviae. Deletion mutagenesis was used to demonstrate that plasmids encoding either the amino or carboxy-terminal regions of the protein, as well as the changing of a single amino acid within IncA (serine 17) could not block C. caviae infection. Immunoblot analysis of truncated IncA in a Vaccinia virus system provided evidence that serine 17 of C. caviae IncA is a target for phosphorylation. These experiments provide insight into the interaction of Inc proteins with the host cell and introduce a model system where these interactions can be explored further.
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