Identification of the gut microbiota biomarkers associated with heat cycle and failure to enter oestrus in gilts.
Identification of the gut microbiota biomarkers associated with heat cycle and failure to enter oestrus in gilts.
复制标题
鉴定与后备母猪发情循环和未能进入发情相关的肠道微生物群生物标志物。
DOI：
10.1111/1751-7915.13695
复制
发表时间：
2021-07
影响因子：
5.7
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Wang Z;Fu H;Zhou Y;Yan M;Chen D;Yang M;Xiao S;Chen C;Huang L
我们在163只后备母猪中发现了与未进入青春期有关的细菌生物特征。对肠道微生物群功能能力的预测发现，在未经历青春期的后备母猪中，视黄醇代谢途径的丰度较低。相关分析表明，肠道细菌的改变可能导致视黄醇代谢紊乱，进而导致无法进入发情期。未成熟的青春期是把后备母猪从生产畜群中剔除的主要原因之一。这通常是由性激素紊乱引起的。越来越多的研究表明，肠道微生物群可能调节性激素，反之亦然。肠道菌群是否与后备母猪的发情失败有关尚不清楚。我们使用16S rRNA基因测序、基于网络的微生物群分析和来自16S rRNA基因序列的功能预测来探索22只8月龄后备母猪在热循环过程中肠道微生物群的变化。我们发现一个由球形毛藻和密螺旋体组成的共现网络模块，在一个热循环中与发情共现。该模块反映网络稳定性和重要性的mcode评分在发情期达到最高值。然后，我们在163只后备母猪中鉴定了与未能显示青春期有关的细菌生物特征。热循环正常的后备母猪中富含普雷沃菌、螺旋体、粪杆菌、尿杆菌、丁二弧菌和厌氧弧菌，而未进入青春期后备母猪中富含毛螺科、瘤胃球菌、粪球菌和示波螺旋菌。对肠道微生物群功能能力的预测发现，在未经历青春期的后备母猪中，“视黄醇代谢”途径的丰度较低。该途径也与本研究中发现的与青春期失败相关的细菌类群显著相关（P < 0.05）。这一结果表明，肠道细菌的改变可能导致视黄醇代谢紊乱，这可能是无法进入发情期的一个解释。
We identified bacterial biosignatures associated with the failure to show puberty in 163 gilts. Prediction of functional capacity of the gut microbiome identified a less abundance of the pathway ‘retinol metabolism’ in gilts that failed to undergo puberty. Correlation analysis suggests that the changed gut bacteria might lead to a disorder of retinol metabolism, and further result in the failure to enter oestrus. Failed puberty is one of the main reasons for eliminating gilts from production herds. This is often caused by disorders of sex hormones. An increasing number of studies have suggested that the gut microbiota may regulate sex hormones and vice versa. Whether the gut microbiota is involved in the failure of oestrus in gilts remains unknown. We used 16S rRNA gene sequencing, network‐based microbiota analysis and prediction of functional capacity from 16S rRNA gene sequences to explore the shifts in the gut microbiota throughout a heat cycle in 22 eight‐month‐old gilts. We found that a module of co‐occurrence networks composed of Sphaerochaeta and Treponema, co‐occurred with oestrus during a heat cycle. The mcode score of this module reflecting the stability and importance in the network achieved the highest value at the oestrus stage. We then identified bacterial biosignatures associated with the failure to show puberty in 163 gilts. Prevotella, Treponema, Faecalibacterium, Oribacterium, Succinivibrio and Anaerovibrio were enriched in gilts showing normal heat cycles, while Lachnospiraceae, Ruminococcus, Coprococcus and Oscillospira had higher abundance in gilts failing to show puberty. Prediction of functional capacity of the gut microbiome identified a lesser abundance of the pathway ‘retinol metabolism’ in gilts that failed to undergo puberty. This pathway was also significantly associated with those bacterial taxa involved in failed puberty identified in this study (P < 0.05). This result suggests that the changed gut bacteria might result in a disorder of retinol metabolism, and this may be an explanation for the failure to enter oestrus.
登录
查看更多内容
影响因子：
4.2
作者：
Huda, M. Nazmul;Ahmad, Shaikh M.;Stephensen, Charles B.
通讯作者：
Stephensen, Charles B.
影响因子：
2.7
作者：
Clark, DT;Soory, M
通讯作者：
Soory, M
影响因子：
2.5
作者：
Brown, JA;Eberhardt, DM;Godkin, JD
通讯作者：
Godkin, JD
影响因子：
5.9
作者：
FAITH, DP
通讯作者：
FAITH, DP
影响因子：
6.7
作者：
Fauser, Bart C. J. M.;Tarlatzis, Basil C.;Barnhart, Kurt
通讯作者：
Barnhart, Kurt