Promoter methylation of leukemia inhibitory factor receptor gene in colorectal carcinoma.
Promoter methylation of leukemia inhibitory factor receptor gene in colorectal carcinoma.
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DOI：
10.3892/ijo.2011.1050
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发表时间：
2011-08
影响因子：
5.2
通讯作者：
Kim MS
中科院分区：
文献类型：
--
作者：
Cho YG;Chang X;Park IS;Yamashita K;Shao C;Ha PK;Pai SI;Sidransky D;Kim MS
基因启动子的异常甲基化和相应的基因表达缺失在结直肠癌的发生和发展中起着至关重要的作用。IL-6型细胞因子受体，白血病抑制因子受体（LIFR），是多功能细胞因子如LIF的细胞表面受体复合物的组分。在此，我们报告，LIFR甲基化在人类结肠癌。LIFR基因启动子甲基化在肿瘤组织中的发生率较高（65%，52/80）。定量甲基化特异性PCR（TaqMan-MSP）显示，与正常结肠组织相比，原发性结肠直肠癌组织中的LIFR启动子甲基化差异（5%，4/80）。LIFR甲基化在13个正常结肠粘膜样品中未检测到，这些样品来自非癌症患者。LIFR mRNA在结肠癌组织中的表达明显低于相应的正常组织。在所有非恶性的正常和邻近的正常结肠粘膜组织中观察到LIFR蛋白的强表达，而在原发性肿瘤中观察到下调的LIFR蛋白表达。这些结果表明，癌症特异性甲基化和LIFR表达的特异性降低是结肠癌发展中常见的失活事件。
Aberrant methylation of gene promoters and corresponding loss of gene expression plays a critical role in the initiation and progression of colorectal cancer. An IL-6-type cytokine receptor, leukemia inhibitory factor receptor (LIFR), is a component of cell-surface receptor complexes for multi-functional cytokines such as LIF. Herein, we report that LIFR is methylated in human colon cancer. LIFR promoter was methylated in primary tumor tissues with high frequency (65%, 52/80). Quantitative methylation-specific PCR (TaqMan-MSP) demonstrated differential promoter methylation of LIFR in primary colorectal cancer tissues as compared to normal colon tissues (5%, 4/80). LIFR methylation was not detectable in 13 normal colon mucosa samples obtained from patients without cancer. The mRNA expression of LIFR was significantly down-regulated in colon cancer tissues as compared to corresponding normal tissues. A strong expression of LIFR protein was observed in all non-malignant normal and adjacent normal colon mucosa tissues whereas down-regulated LIFR protein expression was observed in primary tumors. These results demonstrate that cancer-specific methylation and a specific decrease of LIFR expression are a common inactivation event in colon cancer development.
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影响因子：
8
作者：
Douglas, AM;Goss, GA;Begley, CG
通讯作者：
Begley, CG
DOI：
10.1073/pnas.95.20.11891
发表时间：
1998-09-29
影响因子：
11.1
作者：
Belinsky, SA;Nikula, KJ;Herman, JG
通讯作者：
Herman, JG
影响因子：
8
作者：
Kim, M. S.;Chang, X.;Sidransky, D.
通讯作者：
Sidransky, D.
影响因子：
56.9
作者：
NAIR, BC;DEVICO, AL;SARNGADHARAN, MG
通讯作者：
SARNGADHARAN, MG
影响因子：
7.3
作者：
Royuela, M;Ricote, M;De Miguel, MP
通讯作者：
De Miguel, MP