Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies.
Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies.
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生物活性水凝胶底物：在平行板流室研究中探测白细胞受体 - 配体相互作用。
DOI：
10.1007/s10439-006-9173-x
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发表时间：
2006-11
影响因子：
3.8
通讯作者：
West, Jennifer L.
中科院分区：
文献类型：
--
作者：
Taite, Lakeshia J.;Rowland, Maude L.;Ruffino, Katie A.;Smith, Bryan R. E.;Lawrence, Michael B.;West, Jennifer L.
活化的整合素在白细胞表面的结合促进炎症期间白细胞与血管内皮的粘附。选择素及其配体之间的相互作用介导滚动，并被认为在白细胞粘附中起重要作用，尽管生理相互作用所需的最小识别基序尚不清楚。我们已经开发了一种新的系统，使用聚（乙二醇）（PEG）水凝胶修饰的整合素结合肽序列或选择素配体唾液酸刘易斯X（SLeX）内的平行板流动室检查白细胞粘附到特定的配体的动力学。以甘氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）、亮氨酸-天冬氨酸-缬氨酸（LDV）和唾液酸基刘易斯X（Lewis X）为配体，将其结合到光聚合PEG二丙烯酸酯水凝胶表面。在平行板流动室中以生理剪切速率灌注在这些凝胶上的白细胞表现出滚动和牢固的粘附，这取决于与水凝胶基质结合的配体的身份和浓度。这种新系统为研究白细胞和内皮细胞之间的相互作用提供了一个独特的基于聚合物的模型，也为开发心血管组织工程的改进支架提供了一个平台。
The binding of activated integrins on the surface of leukocytes facilitates the adhesion of leukocytes to vascular endothelium during inflammation. Interactions between selectins and their ligands mediate rolling, and are believed to play an important role in leukocyte adhesion, though the minimal recognition motif required for physiologic interactions is not known. We have developed a novel system using poly(ethylene glycol) (PEG) hydrogels modified with either integrin-binding peptide sequences or the selectin ligand sialyl Lewis X (SLeX) within a parallel plate flow chamber to examine the dynamics of leukocyte adhesion to specific ligands. The adhesive peptide sequences arginine–glycine–aspartic acid–serine (RGDS) and leucine–aspartic acid–valine (LDV) as well as sialyl Lewis X were bound to the surface of photopolymerized PEG diacrylate hydrogels. Leukocytes perfused over these gels in a parallel plate flow chamber at physiological shear rates demonstrate both rolling and firm adhesion, depending on the identity and concentration of ligand bound to the hydrogel substrate. This new system provides a unique polymer-based model for the study of interactions between leukocytes and endothelium as well as a platform to develop improved scaffolds for cardiovascular tissue engineering.
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影响因子：
6.2
作者：
Lin-Gibson, S;Bencherif, S;Washburn, NR
通讯作者：
Washburn, NR
影响因子：
--
作者：
Gonzalez, AL;Gobin, AS;Smith, CW
通讯作者：
Smith, CW
DOI：
10.1111/j.1749-6632.2002.tb03056.x
发表时间：
2002-01-01
期刊：
REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS
影响因子：
--
作者：
Griffith, LG
通讯作者：
Griffith, LG
影响因子：
20.3
作者：
Evangelista, V;Manarini, S;Cerletti, C
通讯作者：
Cerletti, C
影响因子：
4.8
作者：
Gobin, AS;West, JL
通讯作者：
West, JL