Dynamic SUMO remodeling drives a series of critical events during the meiotic divisions in Caenorhabditis elegans.
Dynamic SUMO remodeling drives a series of critical events during the meiotic divisions in Caenorhabditis elegans.
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DOI：
10.1371/journal.pgen.1007626
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发表时间：
2018-09
期刊：
影响因子：
4.5
通讯作者：
Wignall SM
中科院分区：
文献类型：
--
作者：
Davis-Roca AC;Divekar NS;Ng RK;Wignall SM
C.染色体的聚集和分离。线虫卵母细胞依赖于一种保守蛋白质的复合物，在前中期，这种蛋白质在每个二价体的中心周围形成一个环;然后，这些复合物在分裂后期开始时从染色体上除去，并随着分裂后期的进行而分解。在这里，我们揭示了这些环复合物（RC）的动态调节机制，揭示了蛋白质复合物可以在细胞过程中逐步重塑的策略。我们发现RC的组装、维持和稳定性受SUMO结合和去结合活性之间的平衡调节。在前中期，SUMO蛋白酶ULP-1靶向RC，但被SUMO E2/E3酶抵消;然后在后期早期，E2/E3酶被去除，使ULP-1能够触发RC解体并完成减数分裂。此外，我们发现SUMO调节对于将RC正确连接到染色体以及在后期完全释放RC是必不可少的。总而言之，我们的工作表明，SUMO修饰的动态重塑促进了关键的减数分裂事件，并强调了缀合和去缀合活性之间的竞争如何调节SUMO稳态，蛋白质复合物稳定性，并最终调节细胞分裂等渐进过程。大多数细胞有两套染色体，一套来自父母。减数分裂是细胞分裂的一种特殊形式，其中染色体复制一次并分离两次，以产生每个染色体只有一个副本的卵子（卵母细胞）或精子。这是必要的，这样受精将产生一个胚胎，再次包含两个完整的基因组拷贝。利用C.作为一个模型系统，我们已经发现了重要的调控机制，这些减数分裂的保真度。C.秀丽线虫卵母细胞使用依赖于定位于染色体的大蛋白复合物的不依赖于着丝粒的染色体分离机制。这些蛋白质复合物在中期促进染色体聚集，然后在后期从染色体上释放，并随着染色体分离而逐渐分解。我们发现，这些复合物的稳定性和拆卸是由蛋白质修饰称为SUMO和酶之间的竞争，本地化的蛋白质复合物添加或删除SUMO修饰。这些发现提供了深入了解SUMO和SUMO酶通过细胞分裂调节进展的机制，并说明了一种通用策略，通过该策略，大型蛋白质复合物可以在动态细胞过程中快速组装和分解。
Chromosome congression and segregation in C. elegans oocytes depend on a complex of conserved proteins that forms a ring around the center of each bivalent during prometaphase; these complexes are then removed from chromosomes at anaphase onset and disassemble as anaphase proceeds. Here, we uncover mechanisms underlying the dynamic regulation of these ring complexes (RCs), revealing a strategy by which protein complexes can be progressively remodeled during cellular processes. We find that the assembly, maintenance, and stability of RCs is regulated by a balance between SUMO conjugating and deconjugating activity. During prometaphase, the SUMO protease ULP-1 is targeted to the RCs but is counteracted by SUMO E2/E3 enzymes; then in early anaphase the E2/E3 enzymes are removed, enabling ULP-1 to trigger RC disassembly and completion of the meiotic divisions. Moreover, we found that SUMO regulation is essential to properly connect the RCs to the chromosomes and then also to fully release them in anaphase. Altogether, our work demonstrates that dynamic remodeling of SUMO modifications facilitates key meiotic events and highlights how competition between conjugation and deconjugation activity can modulate SUMO homeostasis, protein complex stability, and ultimately, progressive processes such as cell division. Most cells have two sets of chromosomes, one from each parent. Meiosis is a specialized form of cell division where chromosomes are duplicated once and segregated twice, in order to generate eggs (oocytes) or sperm with only one copy of every chromosome. This is necessary so that fertilization will produce an embryo that once again contains two complete copies of the genome. Using C. elegans as a model system, we have uncovered regulatory mechanisms important for the fidelity of these meiotic divisions. C. elegans oocytes use a kinetochore-independent chromosome segregation mechanism that relies on a large protein complex that localizes to the chromosomes. These protein complexes facilitate chromosome congression during metaphase and then are released from chromosomes in anaphase and progressively disassemble as the chromosomes segregate. We find that the stability and disassembly of these complexes is regulated by a protein modification called SUMO and by competition between enzymes that localize to the protein complex to either add or remove SUMO modifications. These findings provide insight into the mechanisms by which SUMO and SUMO enzymes regulate progression through cell division and illustrate a general strategy by which large protein complexes can be rapidly assembled and disassembled during dynamic cellular processes.
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DOI：
10.1083/jcb.200212052
发表时间：
2003-03-31
期刊：
The Journal of cell biology
影响因子：
--
作者：
Li SJ;Hochstrasser M
通讯作者：
Hochstrasser M
影响因子：
7.8
作者：
Azuma, Yoshiaki;Arnaoutov, Alexei;Dasso, Mary
通讯作者：
Dasso, Mary
DOI：
10.1083/jcb.153.6.1209
发表时间：
2001-06-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Oegema K;Desai A;Rybina S;Kirkham M;Hyman AA
通讯作者：
Hyman AA
影响因子：
4
作者：
Collette, Karishma S.;Petty, Emily L.;Csankovszki, Gyorgyi
通讯作者：
Csankovszki, Gyorgyi
影响因子：
3.3
作者：
Hayashi, Michiko;Mlynarczyk-Evans, Susanna;Villeneuve, Anne M.
通讯作者：
Villeneuve, Anne M.