Effects of ethanol and n-butanol on the fluidity of supported lipid bilayers.
Effects of ethanol and n-butanol on the fluidity of supported lipid bilayers.
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DOI：
10.1016/j.chemphyslip.2021.105091
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发表时间：
2021-08
影响因子：
3.4
通讯作者：
Blanchard GJ
中科院分区：
文献类型：
--
作者：
Hossain M;Blanchard GJ
分子如短链醇与哺乳动物质膜的相互作用被认为在麻醉作用中起作用。我们已经研究了浓度依赖性的影响，乙醇和正丁醇的流动性的平面模型脂质双层结构支持云母。支持的模型双层由1，2-二油酰基-sn-磷脂酰胆碱（DOPC），胆固醇和鞘磷脂，和双层形成的囊泡融合挤出单层囊泡（133 nm直径，多分散指数为0.17）。在囊泡沉积期间加入受控量的乙醇和正丁醇。平移扩散常数，获得利用荧光恢复后光漂白（FRAP）测量微米尺度上与作为荧光团的二萘嵌苯。平移扩散常数随乙醇浓度的增加先增大后减小，在乙醇浓度约为100 mg/L时，双层结构发生降解。0.8 M乙醇。由于与磷脂双层成分的相互作用更大，在较低的醇浓度下观察到正丁醇的类似趋势。对于正丁醇，平面双层结构的完整性恶化约。0.4 M正丁醇。这一结果与双层指状结构相一致。
The interactions of molecules such as short-chain alcohols with the mammalian plasma membrane are thought to play a role in anesthetic effects. We have examined the concentration-dependent effects of ethanol and n-butanol on the fluidity of planar model lipid bilayer structures supported on mica. The supported model bilayer was composed of 1,2-dioleoyl-sn-phosphatidylcholine (DOPC), cholesterol, and sphingomyelin, and the bilayers were formed by vesicle fusion from extruded unilamellar vesicles (133 nm diameter, polydispersity index of 0.17). Controlled amounts of ethanol and n-butanol were added during vesicle deposition. Translational diffusion constants were obtained utilizing fluorescence recovery after photobleaching (FRAP) measurements on the micrometer scale with perylene as the fluorophore. The translational diffusion constants increased and then decreased with increasing ethanol concentration, with the bilayer structure degrading at ca. 0.8 M ethanol. A similar trend was observed for n-butanol at lower alcohol concentrations owing to greater interactions with phospholipid bilayer constituents. For n-butanol, the integrity of the planar bilayer structure deteriorated at ca. 0.4 M n-butanol. The results are consistent with bilayer interdigitation.
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DOI：
10.1016/0005-2736(81)90017-1
发表时间：
1981-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
JANOFF, AS;PRINGLE, MJ;MILLER, KW
通讯作者：
MILLER, KW
影响因子：
3.9
作者：
Kijewska, Krystyna;Blanchard, Gary J.
通讯作者：
Blanchard, Gary J.
影响因子：
3.4
作者：
LOBBECKE, L;CEVE, G
通讯作者：
CEVE, G
影响因子：
2.4
作者：
Einstein, A
通讯作者：
Einstein, A
影响因子：
--
作者：
Emmanouil, Dimitris E;Quock, Raymond M
通讯作者：
Quock, Raymond M