Loop-armed DNA tetrahedron nanoparticles for delivering antisense oligos into bacteria
Loop-armed DNA tetrahedron nanoparticles for delivering antisense oligos into bacteria
复制标题
用于将反义寡核苷酸递送到细菌中的环臂DNA四面体纳米颗粒
DOI：
10.1186/s12951-020-00667-6
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发表时间：
2020-03
影响因子：
10.2
通讯作者：
Xue Xiaoyan
中科院分区：
文献类型：
--
作者：
Hu Yue;Chen Zhou;Mao Xinggang;Li Mingkai;Hou Zheng;Meng Jingru;Luo Xiaoxing;Xue Xiaoyan
背景反义寡核苷酸（ASOS）技术被认为是针对抗生素耐药菌的潜在策略。但是，应用ASO的主要障碍是如何有效地将它们传递到细菌中。 DNA四面体（
BackgroundAntisense oligonucleotides (ASOs) based technology is considered a potential strategy against antibiotic-resistant bacteria; however, a major obstacle to the application of ASOs is how to deliver them into bacteria effectively. DNA tetrahedra (Td) is an emerging carrier for delivering ASOs into eukaryotes, but there is limited information about Td used for bacteria. In this research, we investigated the uptake features of Td and the impact of linkage modes between ASOs and Td on gene-inhibition efficiency in bacteria.ResultsTd was more likely to adhere to bacterial membranes, with moderate ability to penetrate into the bacteria. Strikingly, Td could penetrate into bacteria more effectively with the help of Lipofectamine 2000 (LP2000) at a 0.125 μL/μg ratio to Td, but the same concentration of LP2000 had no apparent effect on linear DNA. Furthermore, linkage modes between ASOs and Td influenced gene-knockdown efficiency. Looped structure of ASOs linked to one side of the Td exhibited better gene-knockdown efficiency than the overhung structure.ConclusionsThis study established an effective antisense delivery system based on loop-armed Td, which opens opportunities for developing antisense antibiotics.
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影响因子：
5.3
作者：
通讯作者：
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影响因子：
12.4
作者：
Zhen Zhao;Yuanke Li;R. Shukla;Hao Liu;Akshay Jain;Ashutosh J Barve;K. Cheng
通讯作者：
Zhen Zhao;Yuanke Li;R. Shukla;Hao Liu;Akshay Jain;Ashutosh J Barve;K. Cheng
影响因子：
4.9
作者：
Kim, Kyoung-Ran;Kim, Da-Rae;Ahn, Dae-Ro
通讯作者：
Ahn, Dae-Ro
影响因子：
10.7
作者：
通讯作者：
--
影响因子：
4.6
作者：
Równicki M;Wojciechowska M;Wierzba AJ;Czarnecki J;Bartosik D;Gryko D;Trylska J
通讯作者：
Trylska J