Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells.
Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells.
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DOI：
10.3390/v13112158
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发表时间：
2021-10-26
期刊：
影响因子：
--
通讯作者：
Guo H
中科院分区：
文献类型：
--
作者：
Zheng L;Zhao F;Ru J;Liu L;Wang Z;Wang N;Shu X;Wei Z;Guo H
猪传染性胃肠炎病毒（transmissible gastroenteritis virus，TGEV）是一种引起仔猪肠道疾病的冠状病毒。介孔二氧化硅纳米粒子是一种安全的疫苗佐剂，可以增强免疫应答。我们前期的研究证实了纳米硅对TGEV灭活疫苗具有免疫增强作用。本研究进一步阐明了含MSNs的TGEV灭活疫苗对猪树突状细胞（DCs）的免疫增强机制。我们的结果表明，灭活TGEV疫苗与MSN强烈增强激活的DC。未成熟DC的TLR 3、TLR 5、TLR 7、TLR 9和TLR 10，细胞因子IFN-α、IL-1β、IL-6、IL-12和TNF-α，细胞因子受体CCR-7的表达被表征，并且在具有MSN的灭活TGEV疫苗组中显示其自身显著更高。综上所述，含MSNs的TGEV灭活疫苗对猪DC的表型和功能有影响，这有助于更好地理解其免疫增强机制。
A transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus, causing acute swine enteric disease especially in suckling piglets. Mesoporous silica nanoparticles (MSNs) are safe vaccine adjuvant, which could enhance immune responses. Our previous research confirmed that nano silicon had immune-enhancing effects with inactivated TGEV vaccine. In this study, we further clarified the immune-enhancing mechanism of the inactivated TGEV vaccine with MSNs on porcine dendritic cells (DCs). Our results indicated that the inactivated TGEV vaccine with MSNs strongly enhanced the activation of the DCs. Expressions of TLR3, TLR5, TLR7, TLR9, and TLR10, cytokines IFN-α, IL-1β, IL-6, IL-12, and TNF-α, cytokine receptor CCR-7 of immature DCs were characterized and showed themselves to be significantly higher in the inactivated TGEV vaccine with the MSN group. In summary, the inactivated TGEV vaccine with MSNs has effects on the phenotype and function of porcine DCs, which helps to better understand the immune-enhancing mechanism.
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影响因子：
6.4
作者：
Collin M;Bigley V
通讯作者：
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作者：
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DOI：
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发表时间：
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