Cytolytic toxin production by Staphylococcus aureus is dependent upon the activity of the protoheme IX farnesyltransferase.
Cytolytic toxin production by Staphylococcus aureus is dependent upon the activity of the protoheme IX farnesyltransferase.
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DOI：
10.1038/s41598-017-14110-8
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发表时间：
2017-10-23
影响因子：
4.6
通讯作者：
Massey RC
中科院分区：
文献类型：
--
作者：
Stevens E;Laabei M;Gardner S;Somerville GA;Massey RC
金黄色葡萄球菌是一种医学上重要的病原体，具有在宿主内生存所必需的大量毒力因子，包括产生溶细胞毒素。毒素产生的调节是由Agr群体感应系统介导的，并且是不依赖于Agr的定义不明确的指数后生长期信号。作为最近的全基因组关联研究（GWAS）的一部分，以确定新的基因座，改变溶细胞毒素的表达，在cyoE基因，编码血红素IX法尼基转移酶的多态性，被确定。这种酶对于将血红素加工成电子传递链以用作电子受体是必需的。有趣的是，如果没有这种酶S。金黄色葡萄球菌分泌溶细胞毒素的能力受到抑制，这似乎是通过抑制Agr群体感应系统介导的。我们推测，电子传递的损失是诱导抑制TCA循环的代谢能力的反馈抑制，这与RNA III转录减少，防止合成溶细胞毒素。
Staphylococcus aureus is a medically important pathogen with an abundance of virulence factors that are necessary for survival within a host, including the production of cytolytic toxins. The regulation of toxin production is mediated by the Agr quorum sensing system, and a poorly defined post-exponential growth phase signal independent of Agr. As part of a recent genome wide association study (GWAS) to identify novel loci that alter the expression of cytolytic toxins, a polymorphism in the cyoE gene, which encodes a protoheme IX farnesyltransferase, was identified. This enzyme is essential for processing heme into the electron transport chain for use as an electron acceptor. Interestingly, without this enzyme S. aureus were repressed in their ability to secrete cytolytic toxins, and this appears to be mediated through repression of the Agr quorum sensing system. We hypothesize that the loss of electron transport is inducing feedback inhibition of metabolic capabilities that suppress the TCA cycle, and that this coupled with decreased RNAIII transcription prevents synthesis of cytolytic toxins.
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
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作者：
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