Pulmonary arachidonic acid metabolism following acute exposures to ozone and nitrogen dioxide.-猫眼课题宝

急性暴露于臭氧和二氧化氮后的肺花生四烯酸代谢。

DOI：

10.1080/15287399009531456

发表时间：

1990

期刊：

Journal of toxicology and environmental health

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

Schlesinger,RB;Driscoll,KE;Gunnison,AF;Zelikoff,JT

臭氧(O3)和二氧化氮(NO2)是常见的空气污染物，暴露于这些气体中已被证明会影响肺部的生理、生化和结构。这项研究检测了它们调节肺中花生四烯酸代谢产物(Eico-sanid)的能力。将兔暴露于0.1、0.3或1ppm的臭氧；1、3或10ppm的NO2；或暴露于0.3ppm的臭氧和3ppm的NO2的混合物中，持续2小时。每组动物在每次暴露后立即或24小时处死，接受支气管-肺灌洗。用放射免疫法测定灌洗液中选定的二十烷类化合物。暴露于1ppmO_3后，前列腺素E_2(PGE_2)和F2α(PGF_2α)立即升高。暴露于10ppm的NO2可导致6-酮-前列腺素F1α的抑制，而血栓素B2(TXB_2)在暴露于1ppm的NO2后升高，在暴露3和10ppm后降低。O_3/N_2混合气体导致前列腺素E_2和前列腺素F_2α的协同增加，这种反应似乎是由O_3驱动的。这项研究表明，急性暴露于O3或NO2可以改变肺花生四烯酸代谢，并且对这些氧化剂的反应在数量和质量上都不同。

Ozone (O3) and nitrogen dioxide (NO2) are common air pollutants, and exposure to these gases has been shown to affect pulmonary physiology, biochemistry, and structure. This study examined their ability to modulate arachidonic acid metabolites (eico‐sanoids) in the lungs. Rabbits were exposed for 2h to O3at 0.1, 0.3, or 1 ppm; NO2at 1, 3, or 10 ppm; or to a mixture of 0.3 ppm O3and 3 ppm NO2. Groups of animals sacrificed either immediately or 24 h after each exposure underwent broncho‐pulmonary lavage. Selected eicosanoids were assessed in lavage fluid by radioimmu‐noassay. Increases in prostaglandins E2(PGE2) and F2α(PGF2α) were found immediately after exposure to 1 ppm O3. Exposure to 10 ppm NO2resulted in a depression of 6‐keto‐PCF1α, while thromboxane B2(TxB2) was elevated after exposure to 1 ppm NO2and depressed following 3 and 10 ppm. The O3/NO2mixture resulted in synergistic increases in PGE2and PGF2α, with the response appearing to be driven by O3. This study has demonstrated that acute exposure to either O3or NO2can alter pulmonary arachidonic acid metabolism and that the responses to these oxidants differ, both quantitatively and qualitatively.