Expression of pax6 and sox2 in adult olfactory epithelium.
Expression of pax6 and sox2 in adult olfactory epithelium.
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DOI：
10.1002/cne.22463
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发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Schwob, James E.
中科院分区：
文献类型：
--
作者：
Guo, Zhen;Packard, Adam;Krolewski, Richard C.;Harris, Margaret T.;Manglapus, Glen L.;Schwob, James E.
嗅上皮维持干细胞和祖细胞，这些干细胞和祖细胞支持神经上皮在损伤后的终身重建能力。然而，干细胞的身份-及其调控-仍然定义不清。转录因子Pax6和Sox 2是许多组织（包括大脑）中干细胞的特征。因此，我们评估了Pax6和Sox 2在正常嗅上皮和溴甲烷损伤或嗅球切除术后上皮再生过程中的表达。Sox 2存在于正常上皮的多种细胞中，包括支持细胞、水平基底细胞和一些球状基底细胞。Pax6在所有这些中与Sox 2共表达，但也在导管/腺细胞以及支配项链肾小球的嗅觉神经元中发现。大多数Sox2/Pax6阳性的球状基底细胞都在活跃地循环，但也有一些表达细胞周期蛋白依赖性激酶抑制剂p27 Kip1，推测它们在有丝分裂中处于静止状态。在球状基底细胞中，Sox 2和Pax 6由puronecular多能祖细胞（既不被抗Mash 1也不被抗Neurog 1标记）和神经元定向转运扩增细胞（其表达Mash 1）共表达。然而，Sox2和Pax6仅由少数直接神经元前体（Neurog 1和NeuroD 1表达）表达。这些类别的球状基底细胞的分配Sox 2和Pax6证实了由溴甲烷诱导的损伤的上皮细胞的恢复过程中的转录因子表达的时间分析。每一种Sox 2/Pax6标记的细胞类型都与神经元的诞生相距甚远;因此，抑制它们的分化可能是Sox 2/Pax6在嗅上皮中的作用之一。
The olfactory epithelium maintains stem and progenitor cells that support the neuroepithelium’s life-long capacity to reconstitute after injury. However, the identity of the stem cells – and their regulation – remain poorly defined. The transcription factors Pax6 and Sox2 are characteristic of stem cells in many tissues, including the brain. Therefore, we assessed the expression of Pax6 and Sox2 in normal olfactory epithelium and during epithelial regeneration after methyl bromide lesion or olfactory bulbectomy. Sox2 is found in multiple kinds of cells in normal epithelium, including sustentacular cells, horizontal basal cells, and some globose basal cells. Pax6 is co-expressed with Sox2 in all these, but is also found in duct/gland cells as well as olfactory neurons that innervate necklace glomeruli. Most of the Sox2/Pax6-positive globose basal cells are actively cycling, but some express the cyclin-dependent kinase inhibitor p27Kip1, and are presumably mitotically quiescent. Among globose basal cells, Sox2 and Pax6 are co-expressed by putatively multipotent progenitors (labeled by neither anti-Mash1 nor anti-Neurog1) and neuron-committed transit amplifying cells (which express Mash1). However, Sox2 and Pax6 are expressed by only a minority of immediate neuronal precursors (Neurog1- and NeuroD1-expressing). The assignment of Sox2 and Pax6 to these categories of globose basal cells is confirmed by a temporal analysis of transcription factor expression during the recovery of the epithelium from methyl bromide-induced injury. Each of the Sox2/Pax6-colabeled cell types is at a remove from the birth of neurons; thus, suppressing their differentiation may be among the roles of Sox2/Pax6 in the olfactory epithelium.
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影响因子：
2.7
作者：
Bani-Yaghoub, Mahmud;Tremblay, Roger G.;Sikorska, Marianna
通讯作者：
Sikorska, Marianna
影响因子：
4.6
作者：
Cavallaro, Maurizio;Mariani, Jessica;Nicolis, Silvia K.
通讯作者：
Nicolis, Silvia K.
影响因子：
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作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
影响因子：
25
作者：
Favaro, Rebecca;Valotta, Menella;Nicolis, Silvia K.
通讯作者：
Nicolis, Silvia K.
影响因子：
2.7
作者：
Collinson, JM;Quinn, JC;West, JD
通讯作者：
West, JD