Molecular characterization of two isolates of sweet potato leaf curl virus infecting Ipomoea indica in China
Molecular characterization of two isolates of sweet potato leaf curl virus infecting Ipomoea indica in China
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中国两株侵染籼薯的甘薯曲叶病毒分离株的分子特征
DOI：
10.5897/ajb11.1223
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发表时间：
2011-08
影响因子：
--
通讯作者：
Yan Xie
中科院分区：
文献类型：
--
作者：
Awais Zulfiqar;He Ren;Jie Zhang;Jing Li;Xiaofeng Cui;Yan Xie
从江苏和云南采集了表现黄色花叶和脉黄化症状的牵牛花植株。结果表明，JS 1和Y338分离物的DNA-A片段分别由2827和2801个核苷酸组成，基因组结构与菜豆花叶病毒相似。全序列分析表明，该病毒含有两个病毒体正义ORF（AV 1和AV 2）和四个互补ORF（AC 1、AC 2、AC 3和AC 4）。比较分析表明，JS 1分离物与美国甘薯曲叶病毒（SPLCV）的DNA-A序列同源性为97.0%，Y338分离物与中国甘薯曲叶病毒（SPLCV）的DNA-A序列同源性最高，为97.8%。系统进化分析表明，JS 1与SPLCV-US聚在一起，Y338与SPLCV-CN亲缘关系较近。序列分析表明，分离物JS 1和Y338为SPLCV株系。
Morning glory ( Ipomoea indica ) plants showing yellow mosaic and vein yellowing symptoms were collected from Jiangsu and Yunnan province of China. The amplified DNA-A components of two isolates named JS1 and Y338 were comprised of 2827 and 2801 nucleotides, respectively, in length with genome organization similar to that of begomoviruses. Complete nucleotide sequence revealed the presence of two ORFs (AV1 and AV2) in the virion sense and four ORFs (AC1, AC2, AC3 and AC4) in the complementary sense. Comparison analysis showed that DNA-A sequence of JS1 isolate was closely related to that of sweet potato leaf curl virus (SPLCV) from United States with nucleotide sequence identity of 97.0% and DNA-A of Y338 showed highest sequence identity at 97.8% with an isolate of SPLCV from China. Phylogenetic analysis showed that JS1 clustered together with SPLCV-US and Y338 showed close relationship with SPLCV-CN. Sequence analysis indicated that isolates JS1 and Y338 were strains of SPLCV.
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影响因子：
1.6
作者：
Guo, Wei;Yang, Xiuling;Zhou, Xueping
通讯作者：
Zhou, Xueping
影响因子：
2.7
作者：
Fauquet, CM;Bisaro, DM;Stanley, J
通讯作者：
Stanley, J
影响因子：
--
作者：
Xie, Y;Zhou, XP;Li, GX
通讯作者：
Li, GX
DOI：
--
发表时间：
1999
期刊：
--
影响因子：
--
作者：
P. Lotrakul;R. Valverde
通讯作者：
P. Lotrakul;R. Valverde
影响因子：
3.8
作者：
Ha, C;Coombs, S;Dale, J
通讯作者：
Dale, J