Committed T lymphocyte stem cells of rats. Characterization by surface W3/13 antigen and radiosensitivity.
Committed T lymphocyte stem cells of rats. Characterization by surface W3/13 antigen and radiosensitivity.
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DOI：
10.1084/jem.154.4.1164
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发表时间：
1981-10-01
期刊：
影响因子：
--
通讯作者：
Hunt SV
中科院分区：
文献类型：
--
作者：
Dyer MJ;Hunt SV
T淋巴系干细胞的存在是通过研究大鼠T和B干细胞在膜W3/13抗原的表达和体内对伽玛射线的敏感性的不同而推断出来的。用B细胞和T细胞同种异体表面标志检测长期辐射嵌合体中大鼠骨髓和胎肝的干细胞活性，以鉴定供体细胞的后代。小鼠抗大鼠胸腺细胞的单抗W3/13标记了大约40%的胎肝细胞和60%-70%的幼鼠骨髓细胞(亮40%，暗25%)。在荧光激活的细胞分选机上分离明亮、昏暗和阴性的细胞。胎肝中的B、T淋巴干细胞和骨髓中的B淋巴干细胞均为W3/13亮。W3/13DIM骨髓的T细胞再增殖活性是未分离骨髓的一半以上，但几乎没有B细胞再增殖。在进一步的实验中，通过将带有基因标记的骨髓重新繁殖之前，将宿主大鼠暴露在4.5至10Gy伽马照射下，以确定内源性B和T淋巴干细胞的辐射敏感性。结果取决于嵌合体是在第50天之前还是在第100天之后进行检测。在早期，一种抗辐射的T干细胞被提示，其活性后来减弱。因此，大鼠的承诺T干细胞携带适量的W3/13抗原，与再生B淋巴细胞的干细胞相比，具有更强的抗辐射能力，但永久性嵌合体生成较少。
The existence of stem cells committed to the T lymphoid lineage was deduced from studying how rat T and B stem cells differ in their expression of membrane W3/13 antigen and in their susceptibility in vivo to gamma irradiation. Stem cell activity of rat bone marrow and fetal liver was measured in long-term radiation chimeras using B and T cell alloantigenic surface markers to identify the progeny of donor cells. Monoclonal mouse anti-rat thymocyte antibody W3/13 labeled approximately 40% of fetal liver cells and 60-70% of young rat bone marrow cells (40% brightly, 25% dimly). Bright, dim, and negative cells were separated on a fluorescence-activated cell sorter. All B and T lymphoid stem cells in fetal liver were W3/13 bright, as were B lymphoid stem cells in bone marrow. W3/13 dim bone marrow had over half the T cell repopulating activity of unseparated marrow but gave virtually no B cell repopulation. In further experiments, the radiosensitivity of endogenous B and T lymphoid stem cells was determined by exposing host rats to between 4.5 and 10 Gy of gamma irradiation before repopulation with genetically marked marrow. The results depended on whether chimerism was assayed before day 50 or after day 100. At early times, a radioresistant T stem cell was indicated, whose activity waned later. Thus committed T stem cells of rats carry moderate amounts of W3/13 antigen and are more radioresistant but less permanently chimeragenic than the stem cells that regenerate B lymphocytes.
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大鼠中多能血态干细胞上的Thy-1抗原的证明。
DOI：
10.1084/jem.148.5.1351
发表时间：
1978-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Goldschneider I;Gordon LK;Morris RJ
通讯作者：
Morris RJ
影响因子：
4.3
作者：
SPRENT, J
通讯作者：
SPRENT, J
影响因子：
64.8
作者：
CAMPBELL, DG;WILLIAMS, AF;REID, KBM
通讯作者：
REID, KBM
影响因子：
5.4
作者：
BRIDEAU, RJ;CARTER, PB;WILLIAMS, AF
通讯作者：
WILLIAMS, AF
影响因子：
5.6
作者：
MICKLEM, HS;OGDEN, DA;PAPWORTH, DS
通讯作者：
PAPWORTH, DS