PUF-8 suppresses the somatic transcription factor PAL-1 expression in C. elegans germline stem cells.
PUF-8 suppresses the somatic transcription factor PAL-1 expression in C. elegans germline stem cells.
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DOI：
10.1016/j.ydbio.2011.09.021
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发表时间：
2011-12-01
影响因子：
2.7
通讯作者：
Subramaniam K
中科院分区：
文献类型：
--
作者：
Mainpal R;Priti A;Subramaniam K
PUF家族的RNA结合蛋白是非常保守的转录后调节因子，其控制多种发育过程。梭线虫蛋白PUF-8对于生殖细胞发育的几个方面是必需的，包括生殖系干细胞（GSC）的维持。为了探索其功能的分子机制，我们已经确定了160个生殖细胞表达的mRNA作为PUF-8的潜在靶点。我们为17个mRNA生成GFP：：H2 B-3′ UTR融合体，以测定它们在生殖细胞中的转录后调控。12个转基因在有丝分裂生殖细胞中不表达，并且PUF-8的耗尽导致其中6个在这些细胞中的错误表达。而hip-1的3′ UTR融合蛋白（编码HSP-70相互作用蛋白）的表达依赖于PUF-8。这些结果表明，PUF-8可以调节其目标的表达，无论是消极的，以及积极的。我们研究了PUF-8介导的一种mRNA的转录后控制，即pal-1，其编码负责肌肉发育的同源结构域转录因子。我们的结果表明，PUF-8在体外与pal-1 3 'UTR内的特定序列结合，这些序列对于GSC的转录后抑制至关重要。去除PUF-8导致PAL-1的错误表达，以及生肌启动子HLH-1在生殖细胞中的PAL-1依赖性错误表达。我们提出PUF-8保护GSC免受体细胞分化因子如PAL-1的影响，PAL-1在母体生殖系中产生，但用于胚胎发生。
RNA-binding proteins of the PUF family are well conserved post-transcriptional regulators that control a variety of developmental processes. The C. elegans protein PUF-8 is essential for several aspects of germ cell development including the maintenance of germline stem cells (GSCs). To explore the molecular mechanisms underlying its function, we have identified 160 germline-expressed mRNAs as potential targets of PUF-8. We generated GFP::H2B-3′ UTR fusions for 17 mRNAs to assay their post-transcriptional regulation in germ cells. Twelve transgenes were not expressed in the mitotic germ cells, and depletion of PUF-8 led to misexpression of six of them in these cells. In contrast, the expression of 3′ UTR fusion of hip-1, which encodes the HSP-70 interacting protein, was dependent on PUF-8. These results indicate that PUF-8 may regulate the expression of its targets both negatively as well as positively. We investigated the PUF-8-mediated post-transcriptional control of one mRNA, namely pal-1, which encodes a homeodomain transcription factor responsible for muscle development. Our results show that PUF-8 binds in vitro to specific sequences within pal-1 3′ UTR that are critical for post-transcriptional suppression in GSCs. Removal of PUF-8 resulted in PAL-1 misexpression, and PAL-1-dependent misexpression of the myogenic promoter HLH-1 in germ cells. We propose that PUF-8 protects GSCs from the influence of somatic differentiation factors such as PAL-1, which are produced in the maternal germline but meant for embryogenesis.
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影响因子：
14.9
作者：
Baugh, L. R.;Hill, A. A.;Hunter, Craig P.
通讯作者：
Hunter, Craig P.
影响因子：
2.7
作者：
D'Agostino, I;Merritt, C;Subramaniam, K
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影响因子：
11.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Wickens, M
DOI：
10.1073/pnas.0509260103
发表时间：
2006-03-21
影响因子：
11.1
作者：
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通讯作者：
Herschlag, D