Cell density dependent acid sensitivity in stationary phase cultures of enterohemorrhagic Escherichia coli O157:H7.
Cell density dependent acid sensitivity in stationary phase cultures of enterohemorrhagic Escherichia coli O157:H7.
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肠出血性大肠杆菌 O157:H7 稳定期培养物中细胞密度依赖的酸敏感性。
DOI：
10.1111/j.1574-6968.1999.tb08857.x
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发表时间：
1999
影响因子：
2.1
通讯作者：
Melissa M. Benjamin
中科院分区：
文献类型：
--
作者：
A. Datta;Melissa M. Benjamin
大肠杆菌O 157：H7是出血性结肠炎和溶血性尿毒综合征的病原体，可在高酸性环境中存活。该生物体的耐酸性（通过其在低pH值下存活的能力来测量）取决于测定期间存在的细胞密度。在低细胞密度（</=2 × 10（7）ml（-1））下，约100%的稳定期细胞在37 ℃、pH 2.5的Luria肉汤中存活至少7 h。在相同条件下，高细胞密度（2-5 × 10（9）ml（-1））的相同培养物的敏感性约为1000倍。指数期培养物没有表现出细胞密度效应。在大肠杆菌rpoS突变体（rpoS：：pRR 10）的稳定期培养物中，在高细胞密度下不存在增加的酸敏感性。coliO 157：H7菌株中表达。在其他肠出血性大肠杆菌中也观察到了细胞密度依赖的稳定期培养物的酸敏感性。大肠杆菌和志贺氏菌菌株。革兰氏阳性菌在高细胞密度下不存在酸敏感性增加。
Escherichia coli O157:H7, the causative agent of hemorrhagic colitis and hemolytic uremic syndrome, can survive in a highly acidic environment. The acid resistance of this organism, as measured by its ability to survive in low pH, depended on the density of the cells present during the assay. At low cell densities (</=2 x 10(7) ml(-1)), about 100% of the stationary phase cells survived in Luria broth pH 2.5 at 37 degrees C for at least 7 h. The same cultures at high cell densities (2-5 x 10(9) ml(-1)) were about 1000-fold more sensitive under identical conditions. Exponential phase cultures did not exhibit the cell density effect. The increased acid sensitivity at high cell densities was absent in the stationary phase cultures of a rpoS mutant (rpoS::pRR10) of an E. coli O157:H7 strain. Cell density dependent acid sensitivity of the stationary phase cultures was also observed in other enterohemorrhagic E. coli and Shigella strains. The increased acid sensitivity at high cell densities was absent in Gram-positive organisms.
DOI：
10.1073/pnas.89.24.11978
发表时间：
1992-12-15
影响因子：
11.1
作者：
FANG, FC;LIBBY, SJ;GUINEY, DG
通讯作者：
GUINEY, DG
人胃杀菌屏障：作用机制、相对抗菌活性和饮食影响。
DOI：
10.1093/infdis/159.5.979
发表时间：
1989
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Peterson,WL;Mackowiak,PA;Barnett,CC;Marling-Cason,M;Haley,ML
通讯作者：
Haley,ML