Characterization of Fe deficiency induced RING finger family members in apple species
Characterization of Fe deficiency induced RING finger family members in apple species
复制标题
苹果物种缺铁诱导的环指家族成员的特征
DOI：
10.1016/j.plgene.2019.100209
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Wang Y.
中科院分区：
文献类型：
--
作者：
Sun Q. R.;Li K. T.;Zhang G. F.;Feng Y.;Li D. Y.;Chai X. F.;Wu T.;Zhang X. Z.;Xu X. F.;Han Z. H.;Wang Y.
铁（Fe）是植物的重要元素，缺铁会对植物的生长发育、产量和品质造成不利影响。无名指家族是一个很大的群体，以其在植物非生物胁迫下的作用而闻名。本研究的主要目的是确定苹果品种中对铁缺乏有反应的无名指家族成员。通过分析无名指基因的遗传结构、分子进化和表达水平，探讨缺铁胁迫下无名指基因表达的机制。共鉴定出76个响应铁缺乏的无名指基因。根据结构特征和系统发育关系，将鉴定到的基因分为I、II、III和IV四个亚家族。染色体定位表明，76个无名指基因随机分布在17条染色体上。不同基因成员的遗传结构和编码的氨基酸基序各不相同。15对无名指基因被鉴定为片段重复。此外，我们还利用Circos基因对家苹果与其他蔷薇科基因组的进化关系进行了分析。qRT-PCR结果显示了这些基因在缺铁条件下的表达水平，说明缺铁可以诱导这些基因的表达，从而验证了转录组数据的准确性。这些结果为环指基因家族的进化关系提供了有用的信息，对缺铁具有潜在的重要意义。此外，这将有助于在未来的研究中确定这些无名指基因的功能。
Iron (Fe) is a critical element for plants and Fe deficiency can cause adverse effects on their growth, development, yield and quality. RING finger family is a large group known for their role under abiotic stresses in plants. The main aim of this research work was to identify the RING finger family members that responded to Fe deficiency in apple species. Their genetic structure, molecular evolution, and expression levels were analyzed to study the underlying mechanism of RING finger genes under Fe deficiency stress. A total of 76 RING finger genes that responded to Fe deficiency were identified. Based on their structural characteristics and phylogenetic relationships, the identified genes were divided into four subfamilies: Group I, Group II, Group III and Group IV. Chromosomal localization demonstrated that the 76 RING finger genes were randomly distributed on 17 chromosomes. The genetic structure and amino-acid-motif encoded varied among different gene members. Fifteen pairs of RING finger genes were identified as segmental duplicated. Further, synteny analysis with Circos was performed to examine the evolutionary relationship between Malus domestica and other Rosaceae genomes. The results of qRT-PCR in Malus xiaojinensis showed expression levels of these genes under Fe deficiency, indicating that Fe deficiency could induce the expression of these genes, thus verifying the accuracy of transcriptome data. These results provided useful information about the evolutionary relationship of RING finger gene family, potentially important under Fe deficiency. Moreover, it will help to identify the function of these RING finger genes in future research.
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