Regulation of myosin IIA and filamentous actin during insulin-stimulated glucose uptake in 3T3-L1 adipocytes.
Regulation of myosin IIA and filamentous actin during insulin-stimulated glucose uptake in 3T3-L1 adipocytes.
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DOI：
10.1016/j.yexcr.2013.12.011
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发表时间：
2014-03-10
影响因子：
3.7
通讯作者：
Patel YM
中科院分区：
文献类型：
--
作者：
Stall R;Ramos J;Kent Fulcher F;Patel YM
胰岛素刺激的葡萄糖摄取需要肌球蛋白IIA（MyoIIA）和胰岛素响应性葡萄糖转运蛋白4（GLUT 4）在质膜上的共定位，以实现适当的GLUT 4融合。MyoIIA促进各种细胞类型中的丝状肌动蛋白（F-肌动蛋白）重组。在脂肪细胞中，F-肌动蛋白重组是胰岛素刺激的葡萄糖摄取所必需的。目前尚不清楚MyoIIA是否与F-肌动蛋白相互作用，以调节胰岛素诱导的GLUT 4在质膜上的融合。为了阐明MyoIIA和F-肌动蛋白之间的关系，我们研究了胰岛素刺激后MyoIIA和F-肌动蛋白在质膜上的共定位以及这种相互作用的调节。我们的研究结果表明，MyoIIA和F-肌动蛋白共定位在GLUT 4与质膜融合的胰岛素刺激后的网站。此外，抑制MyoII与blebbistatin受损的F-肌动蛋白定位在质膜。接下来，我们研究了钙在MyoIIA-F-肌动蛋白共定位中的调节作用。降低钙或钙调素水平降低共定位的MyoIIA和F-肌动蛋白在质膜。虽然单独的钙可以易位MyoIIA，但它并不刺激F-肌动蛋白在质膜上的积累。两者合计，我们确定，虽然MyoIIA活性是必需的F-肌动蛋白定位在质膜上，它本身是不足以本地化F-肌动蛋白的质膜。
Insulin stimulated glucose uptake requires the colocalization of myosin IIA (MyoIIA) and the insulin-responsive glucose transporter 4 (GLUT4) at the plasma membrane for proper GLUT4 fusion. MyoIIA facilitates filamentous actin (F-actin) reorganization in various cell types. In adipocytes F-actin reorganization is required for insulin-stimulated glucose uptake. What is not known is whether MyoIIA interacts with F-actin to regulate insulin-induced GLUT4 fusion at the plasma membrane. To elucidate the relationship between MyoIIA and F-actin, we examined the colocalization of MyoIIA and F-actin at the plasma membrane upon insulin stimulation as well as the regulation of this interaction. Our findings demonstrated that MyoIIA and F-actin colocalized at the site of GLUT4 fusion with the plasma membrane upon insulin stimulation. Furthermore, inhibition of MyoII with blebbistatin impaired F-actin localization at the plasma membrane. Next we examined the regulatory role of calcium in MyoIIA-F-actin colocalization. Reduced calcium or calmodulin levels decreased colocalization of MyoIIA and F-actin at the plasma membrane. While calcium alone can translocate MyoIIA it did not stimulate F-actin accumulation at the plasma membrane. Taken together, we established that while MyoIIA activity is required for F-actin localization at the plasma membrane, it alone is insufficient to localize F-actin to the plasma membrane.
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影响因子：
3.7
作者：
Fulcher, F. Kent;Smith, Bethany T.;Russ, Misty;Patel, Yashomati M.
通讯作者：
Patel, Yashomati M.
Rab10 和肌球蛋白-Va 介导脂肪细胞中胰岛素刺激的 GLUT4 储存囊泡易位
DOI：
10.1083/jcb.201111091
发表时间：
2012-08-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen Y;Wang Y;Zhang J;Deng Y;Jiang L;Song E;Wu XS;Hammer JA;Xu T;Lippincott-Schwartz J
通讯作者：
Lippincott-Schwartz J
影响因子：
5.6
作者：
Haviv, Lior;Gillo, David;Bernheim-Groswasser, Anne
通讯作者：
Bernheim-Groswasser, Anne
影响因子：
7.7
作者：
Rea, S;James, DE
通讯作者：
James, DE
影响因子：
7.5
作者：
Bresnick, AR
通讯作者：
Bresnick, AR