Knockout of OsACOS12 caused male sterility in rice
Knockout of OsACOS12 caused male sterility in rice
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OsACOS12 基因敲除导致水稻雄性不育
DOI：
10.1007/s11032-017-0722-9
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Shuangcheng Li
中科院分区：
文献类型：
--
作者：
Ting Zou;Zhiyuan He;Lingyun Qu;Mingxing Liu;Jing Zeng;Yanling Liang;Tao Wang;Dan Chen;Qiao Xiao;Jun Zhu;Yueyang Liang;Qiming Deng;Shiquan Wang;Aiping Zheng;Lingxia Wang;Ping Li;Shuangcheng Li
开花植物花粉发育对雄性生殖成功至关重要。保护花粉免受各种环境胁迫和细菌感染的花粉壁在花粉发育中起着至关重要的作用。花粉壁的形成与孢粉成分的生物合成和运输有关。拟南芥ACOS5编码孢子孢素生物合成所需的酰基辅酶a合成酶5。我们鉴定出ACOS5的水稻同源物为OsACOS12。CRISPR/ cas9介导的OsACOS12基因敲除突变体由于花粉壁形成缺陷而具有完全的雄性不育性。β-葡糖醛酸酶报告基因分析和RNA原位杂交表明，OsACOS12在绒毡层和小孢子中特异性表达。OsACOS12- yfp的亚细胞定位表明，OsACOS12蛋白主要定位于内质网和细胞核。这些结果表明，OsACOS12在花粉壁形成和花粉发育中起着关键和保守的作用，并在水稻育种中具有重要意义。
Pollen development in flowering plants is critical for male reproductive success. The pollen wall that protects the pollen from various environment stresses and bacterial infections plays an essential role in pollen development. The formation of pollen wall is associated with the biosynthesis and transport of sporopollenin components. ACOS5 in Arabidopsis encodes an acyl-CoA synthetase 5 required for sporopollenin biosynthesis. We identified the rice homolog of ACOS5 as OsACOS12. The CRISPR/Cas9-mediated OsACOS12 knockout mutant has complete male sterility due to a defect in pollen wall formation. β-Glucuronidase reporter gene analysis and RNA in situ hybridization indicated that OsACOS12 was specifically expressed in tapetum and microspores. The subcellular localization of OsACOS12-YFP demonstrated that OsACOS12 protein was primarily localized in the endoplasmic reticulum and nucleus. Our results suggest that OsACOS12 plays a critical and conserved role in pollen wall formation and pollen development and has implications in rice breeding.
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OsACOS12 是拟南芥酰基辅酶 A 合成酶 5 的直系同源物，在水稻花粉外壁形成和花药发育中发挥重要作用
影响因子：
5.3
作者：
Li Y;Li D;Guo Z;Shi Q;Xiong S;Zhang C;Zhu J;Yang Z
通讯作者：
Yang Z
影响因子：
14.9
作者：
Goujon M;McWilliam H;Li W;Valentin F;Squizzato S;Paern J;Lopez R
通讯作者：
Lopez R
影响因子：
6.4
作者：
Wang, Yanbing;Lin, Ying-Chen;Lo, Clive
通讯作者：
Lo, Clive
DOI：
10.1111/j.1469-8137.2008.02534.x
发表时间：
2008-09
期刊：
The New phytologist
影响因子：
--
作者：
Clarice de Azevedo Souza;Brad Barbazuk;S. Ralph;J. Bohlmann;B. Hamberger;C. Douglas
通讯作者：
Clarice de Azevedo Souza;Brad Barbazuk;S. Ralph;J. Bohlmann;B. Hamberger;C. Douglas
影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
Sumner, Lloyd W.