Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species.
Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species.
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DOI：
10.3390/microorganisms10050959
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发表时间：
2022-05-02
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
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Halorhodspira(HLR.)在所有紫色细菌中，物种是最嗜盐和最亲碱的。HLR。在光养生物中，卤氯菌表现出最低的LH1 Qy跃迁能，是已知的唯一三重极端嗜氧性光生生物，表现出嗜热、嗜盐和嗜碱的表型。最近，我们报道了HLR的异常光谱性质是由静电引起的。卤氯菌LH1复合体。在本工作中，我们考察了盐和pH对来自HLR的LH1-RCS的光谱性质和热稳定性的影响。卤代氯与其中温对应物HLR进行了比较。阿布德尔马利基。在不同盐度或不同pH条件下的实验表明，LH1-RCS在高盐浓度和/或碱性pH条件下的热稳定性基本保持不变，但随着盐浓度和/或pH的降低，其热稳定性明显降低。根据LH1多肽的氨基酸序列及其酸碱性残基的组成和阳离子/阴离子物种的Hofmeister系列，讨论了静电电荷在稳定HLR中的重要性。盐氯菌LH1-RC复合体，使其能够在温暖、高盐和碱性的生境中进行光合作用。
Halorhodospira (Hlr.) species are the most halophilic and alkaliphilic of all purple bacteria. Hlr. halochloris exhibits the lowest LH1 Qy transition energy among phototrophic organisms and is the only known triply extremophilic anoxygenic phototroph, displaying a thermophilic, halophilic, and alkaliphilic phenotype. Recently, we reported that electrostatic charges are responsible for the unusual spectroscopic properties of the Hlr. halochloris LH1 complex. In the present work, we examined the effects of salt and pH on the spectroscopic properties and thermal stability of LH1-RCs from Hlr. halochloris compared with its mesophilic counterpart, Hlr. abdelmalekii. Experiments in which the photocomplexes were subjected to different levels of salt or variable pH revealed that the thermal stability of LH1-RCs from both species was largely retained in the presence of high salt concentrations and/or at alkaline pH but was markedly reduced by lowering the salt concentration and/or pH. Based on the amino acid sequences of LH1 polypeptides and their composition of acidic/basic residues and the Hofmeister series for cation/anion species, we discuss the importance of electrostatic charge in stabilizing the Hlr. halochloris LH1-RC complex to allow it to perform photosynthesis in its warm, hypersaline, and alkaline habitat.
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影响因子：
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