Longitudinal neural and vascular recovery following ultraflexible neural electrode implantation in aged mice.
Longitudinal neural and vascular recovery following ultraflexible neural electrode implantation in aged mice.
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超柔性神经电极植入老年小鼠后的纵向神经和血管恢复。
DOI：
10.1016/j.biomaterials.2022.121905
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发表时间：
2022-12
期刊：
影响因子：
14
通讯作者：
中科院分区：
文献类型：
--
作者：
柔性神经电极通过促进组织电极整合来改善单个神经元的记录寿命和质量。然而，柔性电极的皮质内植入不可避免地引起组织损伤。了解皮质内植入后的纵向神经和血管恢复对于柔性电极在健康和紊乱大脑中不断增长的应用至关重要。老年动物特别令人感兴趣，因为它们在神经系统疾病建模中起着关键作用，但它们的组织电极界面仍然大多未被研究。在这里，我们整合在体内双光子成像和电生理记录，以确定在老年小鼠植入超柔性神经电极后的时间依赖性神经和血管动力学。我们发现在植入后的前两周内血管生成和血管重塑增加，这与电生理记录检测到的局部场电位和单位活动的快速增加相一致。皮层浅层血管重构先于深层血管重构，其持续时间往往长于神经信号的恢复。植入后6周，血管异常消退，导致血管和微循环正常。基于放电模式和波形的推定细胞分类在快速尖峰中间神经元和锥体神经元中显示出相似的恢复时间过程。这些结果阐明了植入物附近的结构损伤和重塑如何影响记录功效，并支持在对内源性神经生理学干扰最小的情况下将超柔性电极应用于老年动物。
Flexible neural electrodes improve the recording longevity and quality of individual neurons by promoting tissue-electrode integration. However, the intracortical implantation of flexible electrodes inevitably induces tissue damage. Understanding the longitudinal neural and vascular recovery following the intracortical implantation is critical for the ever-growing applications of flexible electrodes in both healthy and disordered brains. Aged animals are of particular interest because they play a key role in modeling neurological disorders, but their tissue-electrode interface remains mostly unstudied. Here we integrate in-vivo two-photon imaging and electrophysiological recording to determine the time-dependent neural and vascular dynamics after the implantation of ultraflexible neural electrodes in aged mice. We find heightened angiogenesis and vascular remodeling in the first two weeks after implantation, which coincides with the rapid increase in local field potentials and unit activities detected by electrophysiological recordings. Vascular remodeling in shallow cortical layers preceded that in deeper layers, which often lasted longer than the recovery of neural signals. By six weeks post-implantation vascular abnormalities had subsided, resulting in normal vasculature and microcirculation. Putative cell classification based on firing pattern and waveform shows similar recovery time courses in fast-spiking interneurons and pyramidal neurons. These results elucidate how structural damages and remodeling near implants affecting recording efficacy, and support the application of ultraflexible electrodes in aged animals at minimal perturbations to endogenous neurophysiology.
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影响因子：
1.2
作者：
Drew PJ;Blinder P;Cauwenberghs G;Shih AY;Kleinfeld D
通讯作者：
Kleinfeld D
影响因子：
14.8
作者：
Holtmaat, Anthony;Bonhoeffer, Tobias;Chow, David K.;Chuckowree, Jyoti;De Paola, Vincenzo;Hofer, Sonja B.;Huebener, Mark;Keck, Tara;Knott, Graham;Lee, Wei-Chung A.;Mostany, Ricardo;Mrsic-Flogel, Tom D.;Nedivi, Elly;Portera-Cailliau, Carlos;Svoboda, Karel;Trachtenberg, Joshua T.;Wilbrecht, Linda
通讯作者：
Wilbrecht, Linda
影响因子：
1.2
作者：
Felix, Sarah H.;Shah, Kedar G.;Pannu, Satinderpall S.
通讯作者：
Pannu, Satinderpall S.
细胞外田地和电流的起源-EEG，ECOG，LFP和尖峰。
DOI：
10.1038/nrn3241
发表时间：
2012-05-18
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Buzsáki G;Anastassiou CA;Koch C
通讯作者：
Koch C
影响因子：
3
作者：
Gray, CM;Maldonado, PE;McNaughton, B
通讯作者：
McNaughton, B