The role of miR-21 in nickel nanoparticle-induced MMP-2 and MMP-9 production in mouse primary monocytes: In vitro and in vivo studies.
The role of miR-21 in nickel nanoparticle-induced MMP-2 and MMP-9 production in mouse primary monocytes: In vitro and in vivo studies.
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DOI：
10.1016/j.envpol.2020.115597
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Mo Y;Zhang Y;Mo L;Wan R;Jiang M;Zhang Q
暴露于金属纳米颗粒会引起肺部和全身的影响。纳米颗粒可以进入血液循环，直接或间接作用于血细胞，如单核细胞。单核细胞/巨噬细胞是最早到达炎症部位的细胞之一，在免疫反应中起着关键作用。本文研究了纳米镍（Nano-Ni）、部分[O]钝化纳米镍（Nano-Ni- p）和碳包覆纳米镍（Nano-Ni- c）对小鼠原代单核细胞MMP-2和MMP-9生成的影响，并探讨了其潜在机制。剂量和时间反应研究表明，野生型（WT）小鼠原代单核细胞暴露于30 μg/mL纳米镍24 h后，MMP-2和MMP-9的产生显著；因此，选择这些剂量和时间点进行以下体外研究。纳米ni和纳米ni - p导致WT小鼠单核细胞miR-21上调，MMP-2、MMP-9、TIMP-1和TIMP-2上调，而miR-21敲除（KO）小鼠不上调，表明miR-21在纳米ni诱导的MMPs和TIMPs上调中起重要作用。然而，纳米ni - c并没有引起这些影响，这表明纳米ni的表面修饰，如碳涂层，减轻了纳米ni诱导的miR-21和MMPs的上调。通过对WT和miR-21 KO小鼠气管内灌注纳米镍颗粒的体内研究进一步证实了这些结果。最后，我们的研究结果表明，WT小鼠的原代单核细胞暴露于Nano-Ni和Nano-Ni- p会导致miR-21的直接靶点RECK的下调，这表明miR-21/RECK通路参与了Nano-Ni诱导的MMP-2和MMP-9的产生。
Exposure to metal nanoparticles causes both pulmonary and systemic effects. Nanoparticles can enter the circulation and act directly or indirectly on blood cells, such as monocytes. Monocytes/macrophages are among the first cells to home to inflammatory sites and play a key role in the immune response. Here we investigated the effects of nickel nanoparticles (Nano-Ni), partially [O]-passivated Nano-Ni (Nano-Ni-P), and carbon-coated Nano-Ni (Nano-Ni-C) on MMP-2 and MMP-9 production in mouse primary monocytes both in vitro and in vivo and explored the potential mechanisms involved. The dose- and time-response studies showed that exposure of primary monocytes from wild-type (WT) mice to 30 μg/mL of Nano-Ni for 24 h caused significant MMP-2 and MMP-9 production; therefore, these dose and time point were chosen for the following in vitro studies. Nano-Ni and Nano-Ni-P caused miR-21 upregulation, as well as MMP-2, MMP-9, TIMP-1 and TIMP-2 upregulation in monocytes from WT, but not miR-21 knock-out (KO), mice, indicating the important role of miR-21 in Nano-Ni-induced MMPs and TIMPs upregulation. However, Nano-Ni-C did not cause these effects, suggesting surface modification of Nano-Ni, such as carbon coating, alleviates Nano-Ni-induced miR-21 and MMPs upregulation. These results were further confirmed by in vivo studies by intratracheal instillation of nickel nanoparticles into WT and miR-21 KO mice. Finally, our results demonstrated that exposure of primary monocytes from WT mice to Nano-Ni and Nano-Ni-P caused downregulation of RECK, a direct miR-21 target, suggesting the involvement of miR-21/RECK pathway in Nano-Ni-induced MMP-2 and MMP-9 production.
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DOI：
10.1186/s12958-018-0364-8
发表时间：
2018-05-11
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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影响因子：
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通讯作者：
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DOI：
10.3390/molecules16108894
发表时间：
2011-10-24
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Galdiero S;Falanga A;Vitiello M;Cantisani M;Marra V;Galdiero M
通讯作者：
Galdiero M