Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study.
Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study.
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DOI：
10.1016/j.dental.2009.11.153
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发表时间：
2010-04
期刊：
影响因子：
5
通讯作者：
Pashley, David H.
中科院分区：
文献类型：
--
作者：
Breschi, Lorenzo;Mazzoni, Annalisa;Nato, Fernando;Carrilho, Marcela;Visintini, Erika;Tjaderhane, Leo;Ruggeri, Alessandra, Jr.;Tay, Franklin R.;Dorigo, Elettra De Stefano;Pashley, David H.
本研究评价了内源性牙本质基质金属蛋白酶在粘接界面胶原纤维自动降解中的作用。检验的零假设是粘合剂混合物或二葡萄糖酸氯己定（CHX）应用不会改变牙本质MMPs活性，并且CHX用作治疗底漆不会随时间推移改善粘合剂界面的稳定性。用Adper Scotchbond 1XT（SB 1XT）处理人牙本质粉末，在未处理牙本质或0.2- 2%CHX处理牙本质上，获得蛋白提取物的酶谱，以测定牙本质MMPs活性。在37°C下在人工唾液中储存2年后立即分析SB 1XT结合界面（在蚀刻表面上有或没有CHX预处理30秒）的微拉伸结合强度和界面纳米泄漏表达。酶谱表明，SB 1XT的应用程序的人牙本质粉末增加MMP-2的活性，而CHX预处理抑制所有牙本质明胶分解活性，无论从测试的浓度。CHX显着降低人工老化2年的酸蚀树脂粘结牙本质的粘结强度和纳米渗漏的损失。该研究证明了SB 1XT在牙本质MMP-2活化中的积极作用和CHX抑制MMP的功效，即使在低浓度（0.2%）下使用。
This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time. Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2–2% CHX treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30s on the etched surface) were analyzed immediately and after 2 yr of storage in artificial saliva at 37°C. Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 yr. The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2%).
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影响因子：
7.6
作者：
Carrilho, M. R. O.;Carvalho, R. M.;Tjaderhane, L.
通讯作者：
Tjaderhane, L.
影响因子：
14
作者：
Mazzoni, Annalisa;Pashley, David H.;Tay, Franklin R.
通讯作者：
Tay, Franklin R.
影响因子：
7.6
作者：
Shono, Y;Ogawa, T;Pashley, DH
通讯作者：
Pashley, DH
影响因子：
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作者：
Martin-De las Heras, S;Valenzuela, A;Overall, CM
通讯作者：
Overall, CM
影响因子：
7.6
作者：
Sulkala, M;Larmas, M;Tjäderhane, L
通讯作者：
Tjäderhane, L