Reversible Immunoaffinity Interface Enables Dynamic Manipulation of Trapping Force for Accumulated Capture and Efficient Release of Circulating Rare Cells.
Reversible Immunoaffinity Interface Enables Dynamic Manipulation of Trapping Force for Accumulated Capture and Efficient Release of Circulating Rare Cells.
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可逆免疫亲和界面可动态操纵捕获力，以累积捕获和有效释放循环稀有细胞
DOI：
10.1002/advs.202102070
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Chen X;Ding H;Zhang D;Zhao K;Gao J;Lin B;Huang C;Song Y;Zhao G;Ma Y;Wu L;Yang C
识别界面的可控组装和拆卸对于生物分析至关重要。在此，提出了一种通过在用于液体活检的人字形芯片（DynarFace-Chip）中设计动态和可逆的免疫亲和微界面（DynarFace）来动态操纵捕获力的策略。DynarFace是通过磁性吸附免疫磁珠在芯片基底上组装而成，具有操作方便、组装可逆等优点。DynarFace允许在流体动力学增强的界面碰撞过程中在循环稀有细胞（CRC）表面上累积IMB的附着，其中累积增强的磁捕获力与传统的非动态界面相比，将对血液样本中生物标志物表达中等的CRC的捕获效率提高了134.81%。此外，磁体撤回诱导的捕获力消失提供DynarFace拆卸和CRC释放，具有高效率（>98%）和高活力（> 98%），与下游体外培养和CRC基因分析相容。这种DynarFace策略为累积捕获和可逆释放CRCs开辟了一条新途径，为基于液体活检的精准医学提供了巨大潜力。在微流控芯片中设计了一种动态可逆的免疫亲和微界面，具有操作简单、组装可逆等优点。它能够动态操纵捕获力，以累积捕获和可逆释放循环稀有细胞，在基于液体活检的精准医学中具有巨大潜力。
Controllable assembly and disassembly of recognition interface are vital for bioanalysis. Herein, a strategy of dynamic manipulation of trapping force by engineering a dynamic and reversible immunoaffinity microinterface (DynarFace) in a herringbone chip (DynarFace‐Chip) for liquid biopsy is proposed. The DynarFace is assembled by magnetically attracting immunomagnetic beads (IMBs) on chip substrate, with merits of convenient operation and reversible assembly. The DynarFace allows accumulating attachment of IMBs on circulating rare cell (CRC) surfaces during hydrodynamically enhanced interface collision, where accumulatively enhanced magnetic trapping force improves capture efficiency toward CRCs with medium expression of biomarkers from blood samples by 134.81% compared with traditional non‐dynamic interfaces. Moreover, magnet withdrawing‐induced disappearance of trapping force affords DynarFace disassembly and CRC release with high efficiency (>98%) and high viability (≈98%), compatible with downstream in vitro culture and gene analysis of CRCs. This DynarFace strategy opens a new avenue to accumulated capture and reversible release of CRCs, holding great potential for liquid biopsy‐based precision medicine. A dynamic and reversible immunoaffinity microinterface is engineered in a microfluidic chip, with merits of easy operation and reversible assembly. It enables dynamic manipulation of trapping force for accumulated capture and reversible release of circulating rare cells, holding great potential in liquid biopsy‐based precision medicine.
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