Visualizing the structural changes of bacteriophage Epsilon15 and its Salmonella host during infection.
Visualizing the structural changes of bacteriophage Epsilon15 and its Salmonella host during infection.
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可视化感染过程中噬菌体Epsilon15及其沙门氏菌宿主的结构变化。
DOI：
10.1016/j.jmb.2010.07.058
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发表时间：
2010-10-01
影响因子：
5.6
通讯作者：
Chiu W
中科院分区：
文献类型：
--
作者：
Chang JT;Schmid MF;Haase-Pettingell C;Weigele PR;King JA;Chiu W
双链DNA噬菌体传递染色体穿过革兰氏阴性菌的外膜、细胞壁和内膜的有效机制仍然不清楚。单粒子冷冻电子显微镜的进展最近揭示了各种噬菌体成熟病毒粒子内DNA注射装置的组织细节，包括ε 15（ε15）和P-SSP 7。我们使用电子冷冻断层扫描和3D子体积平均来捕获感染宿主沙门氏菌的ε15的快照。这些结构表明感染的以下阶段。在第一阶段，ε15的尾刺附着在宿主细胞的表面。接着，ε15的尾毂连接到一个假定的细胞受体上，并建立一个通道，通过该通道，入口后面的注射核心蛋白离开病毒体。一个横跨周质空间的管被形成用于病毒DNA的通过，推测是来自核心蛋白的重排或来自细胞组分。这个管将引导DNA进入细胞质，并保护它免受周质核酸酶的侵害。一旦DNA被注射到细胞中，管和门户就会封闭，空噬菌体就会留在细胞表面。
The efficient mechanism by which double stranded DNA bacteriophages deliver their chromosome across the outer membrane, cell wall, and inner membrane of Gram-negative bacteria remains obscure. Advances in single particle electron cryo-microscopy have recently revealed details of the organization of the DNA injection apparatus within the mature virion for various bacteriophages, including epsilon15 (ε15) and P-SSP7. We have used electron cryo-tomography and 3D subvolume averaging to capture snapshots of ε15 infecting its host Salmonella anatum. These structures suggest the following stages of infection. In the first stage, the tailspikes of ε15 attach to the surface of the host cell. Next ε15's tail hub attaches to a putative cell receptor and establishes a tunnel through which the injection core proteins behind the portal exit the virion. A tube spanning the periplasmic space is formed for viral DNA passage, presumably from the rearrangement of core proteins or from cellular components. This tube would direct the DNA into the cytoplasm and protect it from periplasmic nucleases. Once the DNA has been injected into the cell, the tube and portal seals, and the empty bacteriophage remains at the cell surface.
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影响因子：
3.7
作者：
Chang, Chung-Yu;Kemp, Priscilla;Molineux, Ian J.
通讯作者：
Molineux, Ian J.
影响因子：
5.7
作者：
Aksyuk, Anastasia A.;Leiman, Petr G.;Rossmann, Michael G.
通讯作者：
Rossmann, Michael G.
影响因子：
4.8
作者：
Boulanger, Pascale;Jacquot, Pierre;Letellier, Lucienne
通讯作者：
Letellier, Lucienne
影响因子：
12.3
作者：
Daubin V;Lerat E;Perrière G
通讯作者：
Perrière G
影响因子：
5.4
作者：
Chang, Juan T.;Schmid, Michael F.;Chiu, Wah
通讯作者：
Chiu, Wah