Muscle satellite cells adopt divergent fates: a mechanism for self-renewal?
Muscle satellite cells adopt divergent fates: a mechanism for self-renewal?
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DOI：
10.1083/jcb.200312007
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发表时间：
2004-08-02
期刊：
影响因子：
--
通讯作者：
Beauchamp JR
中科院分区：
文献类型：
--
作者：
Zammit PS;Golding JP;Nagata Y;Hudon V;Partridge TA;Beauchamp JR
成年骨骼肌的生长、修复和再生依赖于卫星细胞的存在：肌肉干细胞位于围绕每个肌纤维的基底层之下。然而，卫星细胞室是如何维持的尚不清楚。在这里，我们使用培养的肌纤维模型肌肉再生，并表明卫星细胞采取不同的命运。静止卫星细胞被同步激活以共表达转录因子Pax 7和MyoD。大多数然后增殖，下调Pax 7，并分化。相反，其他增殖细胞保持Pax 7，但失去MyoD并退出立即分化。这些细胞通常位于集群中，与Pax 7 −ve后代一起分化。一些Pax 7 +ve/MyoD-ve细胞随后离开细胞周期，从而恢复静止卫星细胞表型。值得注意的是，包含在一个集群中的非周期性细胞可以被刺激再次增殖。这些观察结果表明，卫星细胞分化或从终末肌发生切换，以维持卫星细胞池。
Growth, repair, and regeneration of adult skeletal muscle depends on the persistence of satellite cells: muscle stem cells resident beneath the basal lamina that surrounds each myofiber. However, how the satellite cell compartment is maintained is unclear. Here, we use cultured myofibers to model muscle regeneration and show that satellite cells adopt divergent fates. Quiescent satellite cells are synchronously activated to coexpress the transcription factors Pax7 and MyoD. Most then proliferate, down-regulate Pax7, and differentiate. In contrast, other proliferating cells maintain Pax7 but lose MyoD and withdraw from immediate differentiation. These cells are typically located in clusters, together with Pax7−ve progeny destined for differentiation. Some of the Pax7+ve/MyoD−ve cells then leave the cell cycle, thus regaining the quiescent satellite cell phenotype. Significantly, noncycling cells contained within a cluster can be stimulated to proliferate again. These observations suggest that satellite cells either differentiate or switch from terminal myogenesis to maintain the satellite cell pool.
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