E3 ligase Hei10: a multifaceted structure-based signaling molecule with roles within and beyond meiosis.
E3 ligase Hei10: a multifaceted structure-based signaling molecule with roles within and beyond meiosis.
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DOI：
10.1101/gad.240408.114
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发表时间：
2014-05-15
影响因子：
10.5
通讯作者：
Zickler D
中科院分区：
文献类型：
--
作者：
De Muyt A;Zhang L;Piolot T;Kleckner N;Espagne E;Zickler D
人侵袭增强子-10（Hei 10）介导减数分裂重组，在细胞增殖中起重要作用。在这里，De Muyt等人分析了Hei 10在真菌Sordaria的减数分裂和整个性周期中的功能。这些数据表明，Hei 10整合了来自联会复合体、重组复合体和细胞周期的信号，通过SUMO化/泛素化介导重组复合体的程序化组装和拆卸。本研究揭示了Hei 10在减数分裂重组中的调控作用，并为它在减数分裂外的作用提供了新的视角。人侵袭增强子-10（Hei 10）介导减数分裂重组，并且还在细胞增殖中起作用。在这里，我们探讨Hei 10的作用，在整个性周期的真菌Sordaria相对于本地化和无效，环结合，和推定的细胞周期蛋白结合（RXL）结构域突变的影响。Hei 10有三种连续的焦点类型。早期病灶形成沿着联会复合体（SC）的中心区域。在这些位置中的一些位置，取决于其RING和RXL结构域，Hei 10介导两种连续类型的重组复合物的发育和周转，每种重组复合物由特征性扩增的Hei 10焦点区分。整合重组结节的超微结构数据进一步揭示，重组复合物分为三种类型，其中之一对应于交叉重组事件期间或之前的SC形成。最后，Hei 10分别通过其RING和RXL结构域正向和负向调节SUMO沿SC的定位沿着。所提出的研究结果表明，Hei 10整合了来自SC、相关重组复合物和细胞周期的信号，以通过SUMO化/泛素化介导重组复合物的发育和程序化周转/进化。类似的细胞周期连接的组装/拆卸开关可能是Hei 10在中心体/纺锤体极体动力学和相关核运输中的定位和作用的基础。我们认为Hei 10是一种独特的基于结构的信号转导蛋白。
Human enhancer of invasion-10 (Hei10) mediates meiotic recombination and plays important roles in cell proliferation. Here, De Muyt et al. analyzed the function of Hei10 during meiosis and throughout the sexual cycle of the fungus Sordaria. The data suggest that Hei10 integrates signals from the synaptonemal complex, recombination complexes, and the cell cycle to mediate the programmed assembly and disassembly of recombination complexes via SUMOylation/ubiquitination. This study delineates the role of Hei10 in regulating meiotic recombination and provides new perspectives on its role outside meiosis. Human enhancer of invasion-10 (Hei10) mediates meiotic recombination and also plays roles in cell proliferation. Here we explore Hei10’s roles throughout the sexual cycle of the fungus Sordaria with respect to localization and effects of null, RING-binding, and putative cyclin-binding (RXL) domain mutations. Hei10 makes three successive types of foci. Early foci form along synaptonemal complex (SC) central regions. At some of these positions, depending on its RING and RXL domains, Hei10 mediates development and turnover of two sequential types of recombination complexes, each demarked by characteristic amplified Hei10 foci. Integration with ultrastructural data for recombination nodules further reveals that recombination complexes differentiate into three types, one of which corresponds to crossover recombination events during or prior to SC formation. Finally, Hei10 positively and negatively modulates SUMO localization along SCs by its RING and RXL domains, respectively. The presented findings suggest that Hei10 integrates signals from the SC, associated recombination complexes, and the cell cycle to mediate both the development and programmed turnover/evolution of recombination complexes via SUMOylation/ubiquitination. Analogous cell cycle-linked assembly/disassembly switching could underlie localization and roles for Hei10 in centrosome/spindle pole body dynamics and associated nuclear trafficking. We suggest that Hei10 is a unique type of structure-based signal transduction protein.
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影响因子：
4.5
作者：
Nowrousian M;Stajich JE;Chu M;Engh I;Espagne E;Halliday K;Kamerewerd J;Kempken F;Knab B;Kuo HC;Osiewacz HD;Pöggeler S;Read ND;Seiler S;Smith KM;Zickler D;Kück U;Freitag M
通讯作者：
Freitag M
DOI：
10.1093/humrep/den300
发表时间：
2008-12
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Brown PW;Hwang K;Schlegel PN;Morris PL
通讯作者：
Morris PL
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
1.6
作者：
CARPENTER, ATC
通讯作者：
CARPENTER, ATC
影响因子：
5.3
作者：
Jantsch, V;Pasierbek, P;Loidl, J
通讯作者：
Loidl, J