Analysis of MsTERT Gene Expression Profile in Alfalfa (Medicago sativa) Indicates Their Response to Abiotic Stress and Seed Aging.
Analysis of MsTERT Gene Expression Profile in Alfalfa (Medicago sativa) Indicates Their Response to Abiotic Stress and Seed Aging.
复制标题
苜蓿（Medicago sativa）中Mstert基因表达谱的分析表明它们对非生物压力和种子衰老的反应。
DOI：
10.3390/plants12102036
复制
发表时间：
2023-05-19
期刊：
影响因子：
--
通讯作者：
Mao P
中科院分区：
文献类型：
--
作者：
Sun S;Ma W;Mao P
种子老化一直被认为是种子发芽和幼苗生长延迟而导致活力丧失的关键因素，从而限制了干草的生产。许多研究发现，端粒与非生物胁迫和种子活力密切相关。然而，端粒应对非生物胁迫、种子活力的分子机制以及植物端粒动态平衡的维持机制尚不清楚。苜蓿（Medicago sativa）享有“饲料之王”的称号，是世界上种植的重要的乳制品蛋白质饲料。本文研究了苜蓿MsTERT在非生物胁迫和种子老化过程中的分子特征、系统发育关系和基因表达分析。本研究从“中牧1号”紫花苜蓿基因组中鉴定到MsTERT，编码全长3615 bp的编码序列（CDS），包含端粒酶- rna结合域（RBD）和逆转录酶域（RT），编码1024个氨基酸，等电点9.58，相对分子质量138.94 kD。亚细胞定位显示MsTERT主要定位于细胞核和线粒体。表达谱结果表明，MsTERT对盐（100 mmol/L NaCl）和干旱（20% PEG 6000）等胁迫条件均有响应。此外，外源激素IAA、ABA和GA3也可能影响MsTERT的表达。此外，MsTERT对种子老化也有反应。我们的研究结果显示，端粒长度、MsTERT表达和种子发芽率之间存在显著的相关性，表明端粒长度缩短，MsTERT表达随苜蓿种子老化而降低。这些结果为相对端粒长度和/或TERT表达作为种子老化的生物标志物的假设提供了一些证据。虽然这一发现有助于为阐明苜蓿种子活力丧失的分子机制提供新的途径，但MsTERT基因调控种子活力的分子机制还有待进一步研究。
Seed aging is always taken as a crucial factor for vigor loss due to delayed seed germination and seedling growth, which limits hay production. Many studies have found that telomeres are closely related to abiotic stress and seed vigor. However, the molecular mechanism of telomeres’ response to abiotic stress, seed vigor, and the maintenance mechanism of plant telomere homeostasis still remain unclear. Alfalfa (Medicago sativa) enjoys the title of “King of Forage”, and is an important protein forage for the dairy industry as planted in the world. This comprehensive investigation was performed to explore the molecular characterization, phylogenetic relationship, and gene expression analysis of MsTERT under abiotic stress and during seed aging in alfalfa. In this study, MsTERT was identified from the ‘Zhongmu 1’ alfalfa genome and encoded a coding sequence (CDS) of 3615 bp in length, consisting of telomerase- RNA-Binding Domain (RBD) and Reverse Transcriptase (RT) domains, 1024 amino acids, an isoelectric point of 9.58, and a relative molecular mass of 138.94 kD. Subcellular localization showed that MsTERT was mainly localized in the nucleus and mitochondria. The results of the expression profile showed that MsTERT was observed to respond to various stress conditions such as salt (100 mmol/L NaCl) and drought (20% PEG 6000). Furthermore, exogenous hormones IAA, ABA, and GA3 showed the potential to affect MsTERT expression. Additionally, MsTERT also responded to seed aging. Our results revealed a marginal but significant association between relative telomere length, MsTERT expression, and seed germination percentage, suggesting that the length of telomeres was shortened, and expression of MsTERT decreased with alfalfa seed aged. These results provide some evidence for the hypothesis of relative telomere length and/or TERT expression serving as biomarkers of seed aging. Although this finding is helpful to offer a new way to elucidate the molecular mechanism of vigor loss in alfalfa seed, further investigation is required to elucidate the molecular mechanism by which the MsTERT gene regulates seed vigor.
登录
查看更多内容
姜黄素在调节糖尿病缺氧引起的并发症中的主抗氧化剂途径中的潜在作用。
DOI：
10.3390/molecules26247658
发表时间：
2021-12-17
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Ghareghomi S;Rahban M;Moosavi-Movahedi Z;Habibi-Rezaei M;Saso L;Moosavi-Movahedi AA
通讯作者：
Moosavi-Movahedi AA
DOI：
10.1161/atvbaha.109.185546
发表时间：
2009-06-01
影响因子：
8.7
作者：
Haendeler, Judith;Droese, Stefan;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
DOI：
10.1016/j.ijbiomac.2013.09.029
发表时间：
2013-11-01
影响因子：
8.2
作者：
Wang, Shaopu;Dong, Xiaofang;Tong, Jianming
通讯作者：
Tong, Jianming
外源抗坏血酸和谷胱甘肽引发对线粒体结构和功能系统的影响以减轻燕麦种子的老化损伤
影响因子：
5.3
作者：
Xia, Fangshan;Cheng, Hang;Wang, Mingya
通讯作者：
Wang, Mingya
用端粒DNA的端粒酶结构。
影响因子：
64.5
作者：
Jiang J;Wang Y;Sušac L;Chan H;Basu R;Zhou ZH;Feigon J
通讯作者：
Feigon J