Towards a sterile insect technique field release of Anopheles arabiensis mosquitoes in Sudan: irradiation, transportation, and field cage experimentation.
Towards a sterile insect technique field release of Anopheles arabiensis mosquitoes in Sudan: irradiation, transportation, and field cage experimentation.
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DOI：
10.1186/1475-2875-7-65
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发表时间：
2008-04-25
期刊：
影响因子：
3
通讯作者：
El-Sayed B
中科院分区：
文献类型：
--
作者：
Helinski ME;Hassan MM;El-Motasim WM;Malcolm CA;Knols BG;El-Sayed B
本文描述的工作是一项利用昆虫不育技术抑制苏丹北部州疟疾病媒阿拉伯按蚊种群的研究的一部分。此前尚未收集过有关非洲按蚊的辐照和运输的数据，此处报告了在苏丹进行的第一系列尝试。此外，还描述了在接近自然条件下在大型田间笼中进行的实验。蚊子在喀土穆经过辐照，并以成虫形式空运到指定用于未来释放的现场地点（距实验室 400 公里）。野外笼子是通过创造条件良好的休息场所来为实验做好准备的。在野外笼子中研究了（受辐射的）实验室雄性和野外收集的雄性的交配和存活，并进行了两次小规模竞争实验。昆虫的辐照遇到了一些小问题，主要与辐照源附近缺乏饲养设施有关。将成年蚊子小规模运送到释放地点导致死亡率极低（< 6%）。田间笼中的实验表明，交配发生频率很高（即交配一两个晚上后，雌性平均有 60% 的受精率），而实验室饲养的雄性（即六十代）能够以与野生雄性相当的速度对野生雌性进行授精。根据翅膀长度数据，雄性对于交配没有体型偏好。根据交配后的重新捕获，蚊子在笼中的存活情况令人满意，大约 60% 的昆虫在一晚后被重新捕获。由于与少量野生雌性个体产卵相关的问题，仅获得了关于雄性竞争力的有限信息。结论是，尽管条件具有挑战性，但在当前环境下小规模照射和昆虫运输不存在重大障碍。野外笼子适用于实验和研究，以测试受辐射雄性的竞争力。下一个挑战是扩大程序规模以适应释放所需的更多昆虫，并提出了进一步实施这种遗传控制策略的建议。
The work described in this article forms part of a study to suppress a population of the malaria vector Anopheles arabiensis in Northern State, Sudan, with the Sterile Insect Technique. No data have previously been collected on the irradiation and transportation of anopheline mosquitoes in Africa, and the first series of attempts to do this in Sudan are reported here. In addition, experiments in a large field cage under near-natural conditions are described. Mosquitoes were irradiated in Khartoum and transported as adults by air to the field site earmarked for future releases (400 km from the laboratory). The field cage was prepared for experiments by creating resting sites with favourable conditions. The mating and survival of (irradiated) laboratory males and field-collected males was studied in the field cage, and two small-scale competition experiments were performed. Minor problems were experienced with the irradiation of insects, mostly associated with the absence of a rearing facility in close proximity to the irradiation source. The small-scale transportation of adult mosquitoes to the release site resulted in minimal mortality (< 6%). Experiments in the field cage showed that mating occurred in high frequencies (i.e. an average of 60% insemination of females after one or two nights of mating), and laboratory reared males (i.e. sixty generations) were able to inseminate wild females at rates comparable to wild males. Based on wing length data, there was no size preference of males for mates. Survival of mosquitoes from the cage, based on recapture after mating, was satisfactory and approximately 60% of the insects were recaptured after one night. Only limited information on male competitiveness was obtained due to problems associated with individual egg laying of small numbers of wild females. It is concluded that although conditions are challenging, there are no major obstacles associated with the small-scale irradiation and transportation of insects in the current setting. The field cage is suitable for experiments and studies to test the competitiveness of irradiated males can be pursued. The scaling up of procedures to accommodate much larger numbers of insects needed for a release is the next challenge and recommendations to further implementation of this genetic control strategy are presented.
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影响因子：
3
作者：
Knols, Bart G.J.;Njiru, Basilio N.;Killeen, Gerry F.
通讯作者：
Killeen, Gerry F.
影响因子：
2.7
作者：
Taye, A;Hadis, M;Wirtz, RA
通讯作者：
Wirtz, RA
DOI：
10.4269/ajtmh.1974.23.288
发表时间：
1974-01-01
影响因子：
3.3
作者：
LOFGREN, CS;DAME, DA;BALDWIN, KF
通讯作者：
BALDWIN, KF
影响因子：
3
作者：
Helinski, MEH;Parker, AG;Knols, BGJ
通讯作者：
Knols, BGJ
影响因子：
2.1
作者：
Githeko, AK;Adungo, NI;Oloo, JA
通讯作者：
Oloo, JA