Development of a generic PCR detection of 3-acetyldeoxy-nivalenol-, 15-acetyldeoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum Clade.
Development of a generic PCR detection of 3-acetyldeoxy-nivalenol-, 15-acetyldeoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum Clade.
复制标题
DOI：
10.3390/ijms9122495
复制
发表时间：
2008-12
影响因子：
5.6
通讯作者：
Liao, Yu-Cai
中科院分区：
文献类型：
--
作者：
Wang, Jian-Hua;Li, He-Ping;Qu, Bo;Zhang, Jing-Bo;Huang, Tao;Chen, Fang-Fang;Liao, Yu-Cai
禾谷镰刀菌能引起小麦和其他小谷物的赤霉病或赤霉病，产生不同种类的霉菌毒素，对人和家畜有害。脱氧雪腐镰刀菌烯醇、3-乙酰脱氧雪腐镰刀菌烯醇、15-乙酰脱氧雪腐镰刀菌烯醇和雪腐镰刀菌烯醇等B型霉菌毒素是中国等国内外报道的主要镰刀菌毒素。根据毛霉菌毒素生物合成途径中涉及的Tri13基因的结构基因序列，建立了一种预测其化学类型的基因组聚合酶链式反应方法。一对来自Tri13基因的单对引物检测到来自15-AcDON-化学型的583bP片段、来自3-AcDON-化学型的644bP片段和来自NIV产生株的859bP片段。用该方法对中国、尼泊尔、美国和欧洲的镰刀菌进行了鉴定，表明它们的真菌毒素化学型与高效液相或GC/MS化学分析及其他聚合酶链式反应方法所得到的一致。从Tri13基因的高变区扩增出霉菌毒素化学型特异性片段，其中15-AcDON-化学型有3个缺失，3-AcDON-化学型有2个缺失，NIV-产生菌没有缺失。这种聚合酶链式反应分析产生了单一的扩增片段，因此应该比其他基于聚合酶链式反应的分析方法更可靠，因为这些分析方法可能产生假阴性结果。对来自几个不同国家和不同宿主的菌株的结果进一步表明，该方法应该具有全球适用性。这是一种快速、可靠和经济有效的方法，用于鉴定镰刀菌中B型毛霉菌毒素的化学类型和食品安全控制。
Fusarium graminearum clade pathogens cause Fusarium head blight (FHB) or scab of wheat and other small cereal grains, producing different kinds of trichothecene mycotoxins that are detrimental to human and domestic animals. Type B trichothecene mycotoxins such as deoxynivalenol, 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON) and nivalenol (NIV) are the principal Fusarium mycotoxins reported in China, as well as in other countries. A genomic polymerase chain reaction (PCR) to predict chemotypes was developed based on the structural gene sequences of Tri13 genes involved in trichothecene mycotoxin biosynthesis pathways. A single pair of primers derived from the Tri13 genes detected a 583 bp fragment from 15-AcDON-chemotypes, a 644 bp fragment from 3-AcDON-chemotypes and an 859 bp fragment from NIV-producing strains. Fusarium strains from China, Nepal, USA and Europe were identified by this method, revealing their mycotoxin chemotypes identical to that obtained by chemical analyses of HPLC or GC/MS and other PCR assays. The mycotoxin chemotype-specific fragments were amplified from a highly variable region located in Tri13 genes with three deletions for 15-AcDON-chemotypes, two deletions for 3-AcDON-chemotypes and no deletion for NIV-producers. This PCR assay generated a single amplicon and thus should be more reliable than other PCR-based assays that showed the absence or presence of a PCR fragment since these assays may generate false-negative results. The results with strains from several different countries as well as from different hosts further indicated that this method should be globally applicable. This is a rapid, reliable and cost-effective method for the identification of type B trichothecene mycotoxin chemotypes in Fusarium species and food safety controls.
登录
查看更多内容
影响因子：
3.5
作者：
Moss, MO;Thrane, U
通讯作者：
Thrane, U
影响因子：
2.7
作者：
Nicholson, P;Rezanoor, HN;Joyce, D
通讯作者：
Joyce, D
影响因子：
2.7
作者：
Chandler, EA;Simpson, DR;Nicholson, P
通讯作者：
Nicholson, P
DOI：
10.1007/978-3-211-49389-2_2
发表时间：
2007-01-01
期刊：
Fortschritte der Chemie organischer Naturstoffe = Progress in the chemistry of organic natural products. Progres dans la chimie des substances organiques naturelles
影响因子：
--
作者：
Grove, J F
通讯作者：
Grove, J F
影响因子：
1.5
作者：
Ji, L.;Cao, K.;Wang, S.
通讯作者：
Wang, S.