Morphologically defined sub-stages of C. elegans vulval development in the fourth larval stage.
Morphologically defined sub-stages of C. elegans vulval development in the fourth larval stage.
复制标题
DOI：
10.1186/s12861-015-0076-7
复制
发表时间：
2015-06-12
影响因子：
--
通讯作者：
Inoue T
中科院分区：
文献类型：
--
作者：
Mok DZ;Sternberg PW;Inoue T
在第四幼虫（L4）期，秀丽隐杆线虫的外阴细胞经历了广泛的形态发生，并伴有基因表达的变化。外阴发育的这一阶段发生在外阴细胞被充分研究后，目前还没有得到很好的理解，但它可能是一个有用的背景，可以研究在发育过程中如何调节复杂的时间序列事件。然而，对于L4期外阴发育不同阶段的精确描述系统一直缺乏。根据Nomarski显微镜观察的形态学标准，我们定义了L4的10个亚阶段（L4.0 ~ L4.9）。在20°C下确定了每个子级的精确定时。我们还重新检查了几个基因表达标记的表达时间，并将这些亚阶段与外阴和子宫的其他发育事件的时间联系起来。该方案允许L4个体的发育时间以大约一小时的分辨率确定，而无需诉诸时间过程实验。这些明确的发育阶段将使基因表达和其他发育事件的描述更加精确。
During the fourth larval (L4) stage, vulval cells of C. elegans undergo extensive morphogenesis accompanied by changes in gene expression. This phase of vulval development, occurring after the well-studied induction of vulval cells, is not well understood but is potentially a useful context in which to study how a complex temporal sequence of events is regulated during development. However, a system for precisely describing different phases of vulval development in the L4 stage has been lacking. We defined ten sub-stages of L4 based on morphological criteria as observed using Nomarski microscopy (L4.0 ~ L4.9). Precise timing of each sub-stage at 20 °C was determined. We also re-examined the timing of expression for several gene expression markers, and correlated the sub-stages with the timing of other developmental events in the vulva and the uterus. This scheme allows the developmental timing of an L4 individual to be determined at approximately one-hour resolution without the need to resort to time course experiments. These well-defined developmental stages will enable more precise description of gene expression and other developmental events.
登录
查看更多内容
影响因子：
4.6
作者：
Pellegrino, Mark W.;Farooqui, Sarfarazhussain;Hajnal, Alex
通讯作者：
Hajnal, Alex
影响因子：
2.7
作者：
Estes, Kathleen A.;Hanna-Rose, Wendy
通讯作者：
Hanna-Rose, Wendy
影响因子：
4.6
作者：
Dalpé, G;Brown, L;Culotti, JG
通讯作者：
Culotti, JG
影响因子：
2.6
作者：
Inoue, Takao;Sherwood, David R.;Sternberg, Paul W.
通讯作者：
Sternberg, Paul W.
影响因子：
2.7
作者：
Hanna-Rose, W;Han, M
通讯作者：
Han, M