Deuterium and phosphorus nuclear magnetic resonance and fluorescence depolarization studies of functional reconstituted sarcoplasmic reticulum membrane vesicles.
Deuterium and phosphorus nuclear magnetic resonance and fluorescence depolarization studies of functional reconstituted sarcoplasmic reticulum membrane vesicles.
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功能性重建肌浆网膜囊泡的氘磷核磁共振和荧光去极化研究。
DOI：
10.1021/bi00516a040
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Fleischer,S
中科院分区：
文献类型：
--
作者：
Seelig,J;Tamm,L;Hymel,L;Fleischer,S
Joachim Seelig, Lukas Tamm, Lin Hymel， and SidneyFleischer*摘要：用1,2 -二[9,10 -2***** H2]油基-、1,2 -二[2,2 - 2fi2]油基-或1,2 -二[9,10 - 2h2] elaidoyl-i«-甘油-3-磷酸胆碱组成的脂质基质重建了肌浆网膜囊泡的功能形态。在某些制剂中，天然磷脂与单一脂质环境的交换达到99%。Ca2+泵蛋白占这些膜中蛋白质的90%，脂蛋白比从每mol Ca2+泵蛋白的50到130 mol磷脂变化。这种高蛋白质含量与天然肌质网相当（每mol Ca2+泵蛋白约110 mol磷脂），是检测蛋白质对磷脂运动特性影响的有利范围。利用四极回波技术在46 MHz波段获得了氘的核磁共振波谱；在36和121 MHz下记录质子解耦的31P核磁共振谱。氘和磷的光谱具有均匀液晶双分子层的特征。结构相关的核磁共振参数，如剩余氘四极分裂常数Avq和磷的化学屏蔽各向异性，在重组的肌浆网膜中比在无蛋白脂质体中小10-20%。这表明了一个小但均匀的脂质紊乱，可能是为了符合Ca2+泵蛋白的不均匀形状。通过测量磷和氘的自旋晶格弛豫时间（7^），研究了重构肌浆网膜的动力学特性。磷酸基和磷脂酰胆碱的氘化顺式和反式双键的弛豫过程都遵循单一指数，这一结果支持了单一均匀脂质环境的结论，即，对于
Joachim Seelig,* Lukas Tamm, Lin Hymel, and SidneyFleischer* abstract: Sarcoplasmic reticulum membrane vesicles were reconstituted in functional form with a lipid matrix composed of either l, 2-di [9, 10-2***** H2] oleoyl-, 1, 2-di [2, 2-2FI2] oleoyl-, or l, 2-di [9, 10-2H2] elaidoyl-i «-glycero-3-phosphocholine. In some of the preparations, the native phospholipids were exchanged with a single lipid environment to theextent of 99%. The Ca2+ pump protein accounted for> 90% of the proteins in these membranes, and the lipid-to-protein ratio varied from 50 to 130 mol of phospholipid per mol of Ca2+ pump protein. This high protein content is comparable to that in natural sarco-plasmic reticulum (approximately 110 mol of phospholipid per mol of Ca2+ pump protein) and is a favorable range to detect the influence of the protein on the motional characteristics of the phospholipids. Deuterium nuclear magnetic resonance (NMR) spectra were obtained at 46 MHz with the quadrupole echo technique; proton-decoupled 31P NMR spectra were re-corded at 36 and 121 MHz. The deuterium and phosphorus spectra were characteristic of a homogeneous liquid-crystalline bilayer. The structure-related NMR parameters such as the residual deuterium quadrupole splitting constant, Avq, and the phosphorus chemical shielding anisotropy,, were found to be 10-20% smaller in reconstituted sarcoplasmic reticulum membranes than in protein-free liposomes. This indicates a small but uniform disordering of the lipids, presumably to conform to the uneven shape of the Ca2+ pump protein. The dynamic properties of reconstituted sarcoplasmic reticulum membranes were studied by measuring the phosphorus and deuterium spin-lattice relaxation times (7^). The relaxation process followed a single exponential for both the phosphate group and the deuterated cis and trans double bonds of the phosphatidylcholine, a result supporting the conclusion of a single homogeneous lipid environment, ie, the same for
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DOI：
10.1016/0005-2736(80)90031-0
发表时间：
1980-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GOMEZFERNANDEZ, JC;GONI, FM;CHAPMAN, D
通讯作者：
CHAPMAN, D
用 13C 标记的脂质 13C-NMR 光谱研究重建肌浆网 Ca2-ATP 酶。
DOI：
10.1515/bchm2.1977.358.2.865
发表时间：
1977
期刊：
Hoppe-Seyler's Zeitschrift fur physiologische Chemie
影响因子：
--
作者：
W. Stoffel;O. Zierenberg;H. Scheefers
通讯作者：
H. Scheefers
DPH 在人红细胞膜中的旋转扩散有限。
影响因子：
2.9
作者：
P. Glatz
通讯作者：
P. Glatz
磷脂酰胆碱囊泡中的头基构象和脂质-胆固醇关联：31P(1H) 核奥弗豪瑟效应研究。
DOI：
--
发表时间：
1975
影响因子：
11.1
作者：
P. Yeagle;W. Hutton;C. Huang;R. Martin
通讯作者：
R. Martin
由分离的肌浆网和重建的肌浆网形成的定向多层的 31P-NMR 研究。
DOI：
10.1016/0005-2736(81)90214-5
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
McLaughlin,AC;Herbette,L;Blasie,JK;Wang,CT;Hymel,L;Fleischer,S
通讯作者：
Fleischer,S