A transcriptomic-phylogenomic analysis of the evolutionary relationships of flatworms.
A transcriptomic-phylogenomic analysis of the evolutionary relationships of flatworms.
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DOI：
10.1016/j.cub.2015.03.034
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发表时间：
2015-05-18
期刊：
影响因子：
9.2
通讯作者：
Telford, Maximilian J.
中科院分区：
文献类型：
--
作者：
Egger, Bernhard;Lapraz, Francois;Tomiczek, Bartlomiej;Mueller, Steven;Dessimoz, Christophe;Girstmair, Johannes;Skunca, Nives;Rawlinson, Kate A.;Cameron, Christopher B.;Beli, Elena;Todaro, M. Antonio;Gammoudi, Mehrez;Norena, Carolina;Telford, Maximilian J.
扁形动物门（门）的相互关系解决得很差，尽管几十年的形态和分子系统发育研究。最早的分支分支（Catenulida，Macrostomorpha和Polycladida）与其他lophotrochozoans共享螺旋卵裂和内黄卵。卵壳类有原始的螺旋卵裂，但衍生出外黄卵。其他目（杆腔目、原串目、三枝目和近缘目、槽鳞目）都有外黄卵，但彼此之间的亲缘关系不确定。寄生Neodermata的订单出现从一个不确定的位置从这些外黄类。为了解决这些问题，我们对18种扁虫和5种其他后生动物的转录组进行了测序。添加已发表的数据产生了来自涵盖所有主要分支的11个目中的27个扁虫分类群的> 107，000个氨基酸的比对，其中缺失数据小于28%。我们的系统发育分析表明，扁形动物包括两个分支的Catenulida和杆形动物。在小杆亚目中，我们发现最早出现的分支是巨口亚目，而不是多枝亚目。我们发现Lecithoepitheliata不是Neoophora的成员，而是Polycladida的姐妹群，这意味着在Lecithoepitheliata和Neoophora中发现的外黄卵的独立起源。我们解决的最基本的分支euneoophoran taxon. Tricladida，Bothrioplanida，Neodermata组成的一组，似乎已经失去了螺旋卵裂和中心体。我们确定Bothrioplanida作为长期寻求最接近的自由生活的姐妹组的寄生Neodermata。在寄生的命令，我们表明，绦虫更接近吸虫比Monogenea，拒绝Cercomeromorpha的概念。我们的研究结果对理解这一主要门的进化具有重要意义。系统发生组学提供了对扁形动物的相互关系的见解，大口扁形动物是basalmost rhabditophorans Polycladida是Lecithoepitheliata/Prorhynchida Bothrioplanida的姐妹群，是Neodermata的自由生活的姐妹群。Egger等人组装了来自11个目中的27个扁虫分类群的> 107，000个比对氨基酸的具有小于28%的缺失数据的cDNA基因组数据集，覆盖所有主要分支，并以高置信度重建了分辨率良好的树，揭示了几个意想不到的分支。
The interrelationships of the flatworms (phylum Platyhelminthes) are poorly resolved despite decades of morphological and molecular phylogenetic studies. The earliest-branching clades (Catenulida, Macrostomorpha, and Polycladida) share spiral cleavage and entolecithal eggs with other lophotrochozoans. Lecithoepitheliata have primitive spiral cleavage but derived ectolecithal eggs. Other orders (Rhabdocoela, Proseriata, Tricladida and relatives, and Bothrioplanida) all have derived ectolecithal eggs but have uncertain affinities to one another. The orders of parasitic Neodermata emerge from an uncertain position from within these ectolecithal classes. To tackle these problems, we have sequenced transcriptomes from 18 flatworms and 5 other metazoan groups. The addition of published data produces an alignment of >107,000 amino acids with less than 28% missing data from 27 flatworm taxa in 11 orders covering all major clades. Our phylogenetic analyses show that Platyhelminthes consist of the two clades Catenulida and Rhabditophora. Within Rhabditophora, we show the earliest-emerging branch is Macrostomorpha, not Polycladida. We show Lecithoepitheliata are not members of Neoophora but are sister group of Polycladida, implying independent origins of the ectolecithal eggs found in Lecithoepitheliata and Neoophora. We resolve Rhabdocoela as the most basally branching euneoophoran taxon. Tricladida, Bothrioplanida, and Neodermata constitute a group that appears to have lost both spiral cleavage and centrosomes. We identify Bothrioplanida as the long-sought closest free-living sister group of the parasitic Neodermata. Among parasitic orders, we show that Cestoda are closer to Trematoda than to Monogenea, rejecting the concept of the Cercomeromorpha. Our results have important implications for understanding the evolution of this major phylum. Phylogenomics provide insights into the interrelationships of Platyhelminthes Macrostomorpha are the basalmost rhabditophorans Polycladida are sister group of Lecithoepitheliata/Prorhynchida Bothrioplanida are the free-living sister group of Neodermata The interrelationships of the flatworms (phylum Platyhelminthes) are poorly resolved. Egger et al. assembled a phylogenomic dataset of >107,000 aligned amino acids with less than 28% missing data from 27 flatworm taxa in 11 orders covering all major clades and reconstruct a well-resolved tree with high confidence, revealing several unexpected clades.
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影响因子：
4.1
作者：
Martín-Durán JM;Egger B
通讯作者：
Egger B
影响因子：
3.7
作者：
Egger B;Steinke D;Tarui H;De Mulder K;Arendt D;Borgonie G;Funayama N;Gschwentner R;Hartenstein V;Hobmayer B;Hooge M;Hrouda M;Ishida S;Kobayashi C;Kuales G;Nishimura O;Pfister D;Rieger R;Salvenmoser W;Smith J;Technau U;Tyler S;Agata K;Salzburger W;Ladurner P
通讯作者：
Ladurner P
影响因子：
3.3
作者：
Hahn C;Fromm B;Bachmann L
通讯作者：
Bachmann L
影响因子：
4
作者：
Perkins, Elizabeth M.;Donnellan, Steve C.;Whittington, Ian D.
通讯作者：
Whittington, Ian D.
影响因子：
64.8
作者：
Philippe, Herve;Brinkmann, Henner;Copley, Richard R.;Moroz, Leonid L.;Nakano, Hiroaki;Poustka, Albert J.;Wallberg, Andreas;Peterson, Kevin J.;Telford, Maximilian J.
通讯作者：
Telford, Maximilian J.