The identification of six novel proteins with fibronectin or collagen type I binding activity from Streptococcus suis serotype 2
The identification of six novel proteins with fibronectin or collagen type I binding activity from Streptococcus suis serotype 2
复制标题
从猪链球菌血清型 2 中鉴定出六种具有纤连蛋白或 I 型胶原蛋白结合活性的新型蛋白
DOI：
10.1007/s12275-014-4311-x
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发表时间：
2014-11
影响因子：
3
通讯作者：
Lu Chengping
中科院分区：
文献类型：
--
作者：
Li Yue;Ma Zhe;Fan Hongjie;Lu Chengping
猪链球菌是一种引起猪脑膜炎和感染性休克的人畜共患病原体。细菌细胞壁和分泌蛋白通常参与与细胞外基质蛋白（ECM）的相互作用，ECM在致病的初始步骤中起重要作用。在这项研究中，2D SDS-PAGE，基于蛋白质印迹的结合亲和力测量，和微量滴定板结合测定被用于鉴定细胞壁和分泌的蛋白质从S。与纤连蛋白和I型胶原蛋白相互作用的猪。我们从S.猪，包括三种蛋白质（翻译延伸因子G，寡肽结合蛋白OppA前体，和磷酸甘油酸酯β），显示纤连蛋白和胶原I型结合活性。据我们所知，这三种新鉴定的蛋白质没有以前报道的纤连蛋白或I型胶原结合活性。总的来说，本研究的目的是从S.这是首次从猪链球菌中分离到6个新蛋白。与纤连蛋白或I型胶原蛋白相互作用的猪。
Streptococcus suis, a major swine pathogen, is an emerging zoonotic agent that causes meningitis and septic shock. Bacterial cell wall and secreted proteins are often involved in interactions with extracellular matrix proteins (ECMs), which play important roles in the initial steps of pathogenesis. In this study, 2D SDS-PAGE, western blotting-based binding affinity measurements, and microtiter plate binding assays were used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type I. We identified six proteins from S. suis, including three proteins (translation elongation factor G, oligopeptide-binding protein OppA precursor, and phosphoglycerate mutase) that show both fibronectin and collagen type I binding activity. To the best of our knowledge, these three newly identified proteins had no previously reported fibronectin or collagen type I binding activity. Overall, the aim in this study was to identify proteins with ECM binding activity from S. suis and it represents the first report of six new proteins from S. suis that interact with fibronectin or collagen type I.
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影响因子：
4.4
作者：
DelVecchio, Vito G.;Connolly, Joseph P.;Mujer, Cesar V.
通讯作者：
Mujer, Cesar V.
影响因子：
2.2
作者：
Hui Zhang;Zhe Ma;Yue Li;Jun-xi Zheng;L. Yi;H. Fan;Chengping Lu
通讯作者：
Hui Zhang;Zhe Ma;Yue Li;Jun-xi Zheng;L. Yi;H. Fan;Chengping Lu
影响因子：
3.2
作者：
Ju, Cun-Xiang;Gu, Hong-Wei;Lu, Cheng-Ping
通讯作者：
Lu, Cheng-Ping
影响因子：
3.3
作者：
CHANTER, N;JONES, PW;ALEXANDER, TJL
通讯作者：
ALEXANDER, TJL
影响因子：
3.4
作者：
Zhang, Wei;Lu, Cheng Ping
通讯作者：
Lu, Cheng Ping