Breathing fresh air into respiratory research with single-cell RNA sequencing.
Breathing fresh air into respiratory research with single-cell RNA sequencing.
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DOI：
10.1183/16000617.0060-2020
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发表时间：
2020-06-30
期刊：
影响因子：
--
通讯作者：
Reyfman PA
中科院分区：
文献类型：
--
作者：
Alexander MJ;Budinger GRS;Reyfman PA
肺部复杂的细胞异质性对该领域的研究人员提出了独特的挑战。虽然批量RNA测序的使用已经成为系统生物学中普遍存在的技术，但该技术必然会使个体对组织整体转录景观的贡献平均化。单细胞RNA测序（scRNA-seq）提供了与批量RNA测序相当的转录组的稳健的、无偏倚的调查，同时保留了关于细胞异质性的信息。在这项技术开发的短短几年内，scRNA-seq已经被广泛应用于呼吸系统研究，并为肺离子细胞和肺纤维化中促纤维化巨噬细胞群体的发现等令人印象深刻的进步做出了贡献。在这篇综述中，我们讨论了在考虑使用scRNA-seq时的一般技术考虑因素，并研究了领先的研究人员如何应用该技术来获得对呼吸生物学从发育到疾病的新见解。此外，我们还讨论了单细胞技术的发展，重点是空间和多组学方法，这些方法有望推动呼吸研究的持续创新。单细胞RNA测序越来越多地用于呼吸系统研究。这项技术可以以不同的方式用于研究肺的发育、结构和功能，以及从肺纤维化到哮喘再到肺癌的肺部疾病。https://bit.ly/36TdvJT
The complex cellular heterogeneity of the lung poses a unique challenge to researchers in the field. While the use of bulk RNA sequencing has become a ubiquitous technology in systems biology, the technique necessarily averages out individual contributions to the overall transcriptional landscape of a tissue. Single-cell RNA sequencing (scRNA-seq) provides a robust, unbiased survey of the transcriptome comparable to bulk RNA sequencing while preserving information on cellular heterogeneity. In just a few years since this technology was developed, scRNA-seq has already been adopted widely in respiratory research and has contributed to impressive advancements such as the discoveries of the pulmonary ionocyte and of a profibrotic macrophage population in pulmonary fibrosis. In this review, we discuss general technical considerations when considering the use of scRNA-seq and examine how leading investigators have applied the technology to gain novel insights into respiratory biology, from development to disease. In addition, we discuss the evolution of single-cell technologies with a focus on spatial and multi-omics approaches that promise to drive continued innovation in respiratory research. Single-cell RNA sequencing is being used more and more in respiratory research. This technology can be leveraged in different ways in studies of lung development, structure and function, and lung diseases from pulmonary fibrosis to asthma to lung cancer. https://bit.ly/36TdvJT
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
12.3
作者：
Guillaumet-Adkins A;Rodríguez-Esteban G;Mereu E;Mendez-Lago M;Jaitin DA;Villanueva A;Vidal A;Martinez-Marti A;Felip E;Vivancos A;Keren-Shaul H;Heath S;Gut M;Amit I;Gut I;Heyn H
通讯作者：
Heyn H
DOI：
10.1513/pats.200803-025hr
发表时间：
2008-09-15
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Franks, Teri J;Colby, Thomas V;Williams, Mary C
通讯作者：
Williams, Mary C
影响因子：
7
作者：
Foley, Joseph W.;Zhu, Chunfang;West, Robert B.
通讯作者：
West, Robert B.
影响因子：
16.6
作者：
Angelidis, Ilias;Simon, Lukas M.;Schiller, Herbert B.
通讯作者：
Schiller, Herbert B.