Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures.
Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures.
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DOI：
10.1007/978-1-0716-1119-7_4
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发表时间：
2021
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影响因子：
--
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中科院分区：
文献类型：
--
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软骨是一种结缔组织，以多种形式存在，都是脊椎动物骨骼的重要组成部分。作为体内模型的补充，其驻留细胞-软骨细胞的培养是与骨骼发育和成人动态平衡以及软骨发育不良和骨关节炎等人类病理相关的机械性和临床前研究的重要实验模型。生长板和关节软骨细胞都能产生软骨细胞外基质成分，但这两种细胞亚型也有不同的表型特性，这说明它们的组织结构特征、功能和命运不同。因此，根据研究目标，应从生长板或关节软骨建立原代软骨细胞培养。在这里，我们描述了我们实验室使用的分离和培养新生和成年小鼠生长板和关节软骨细胞的方法。这两种方法都涉及人工和酶处理程序，以清除污染组织中的软骨样本，并将软骨细胞作为单细胞悬浮液从软骨基质中释放出来。
Cartilage is a connective tissue presenting in several forms that are all essential components of the vertebrate skeleton. Complementing in vivo models, cultures of its resident cells—chondrocytes—are important experimental models in mechanistic and preclinical studies relevant to skeletal development and adult homeostasis and to such human pathologies as chondrodysplasias and osteoarthritis. Both growth plate and articular chondrocytes produce pancartilaginous extracellular matrix components, but the two cell subtypes also have distinct phenotypic properties that account for different structural features, functions, and fates of their tissues. Based on study goals, primary chondrocyte cultures should therefore be established from either growth plate or articular cartilage. Here, we describe the methods used in our laboratory to isolate and culture growth plate and articular chondrocytes from neonatal and adult mice, respectively. Both methods involve manual and enzymatic procedures to clean cartilage samples from contaminating tissues and to release chondrocytes as single-cell suspensions from their cartilage matrix.
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影响因子：
2
作者：
Warman, Matthew L.;Cormier-Daire, Valerie;Hall, Christine;Krakow, Deborah;Lachman, Ralph;LeMerrer, Martine;Mortier, Geert;Mundlos, Stefan;Nishimura, Gen;Rimoin, David L.;Robertson, Stephen;Savarirayan, Ravi;Sillence, David;Spranger, Juergen;Unger, Sheila;Zabel, Bernhard;Superti-Furga, Andrea
通讯作者：
Superti-Furga, Andrea
影响因子：
7.2
作者：
Long, Fanxin;Ornitz, David M.
通讯作者：
Ornitz, David M.
DOI：
10.1016/s1357-2725(02)00301-1
发表时间：
2003-04-01
影响因子：
4
作者：
Archer, CW;Francis-West, P
通讯作者：
Francis-West, P
影响因子：
2.8
作者：
Santoro, Anna;Conde, Javier;Gualillo, Oreste
通讯作者：
Gualillo, Oreste
影响因子：
6.9
作者：
LEFEBVRE, V;GAROFALO, S;DECROMBRUGGHE, B
通讯作者：
DECROMBRUGGHE, B