Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double-network hydrogel.
Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double-network hydrogel.
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DOI：
10.1186/1471-2474-12-49
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发表时间：
2011-02-22
影响因子：
2.3
通讯作者：
Gong JP
中科院分区：
文献类型：
--
作者：
Yokota M;Yasuda K;Kitamura N;Arakaki K;Onodera S;Kurokawa T;Gong JP
关节骨软骨缺损的功能性修复不仅是膝关节外科领域的一大挑战，也是组织再生医学领域的一大挑战。目的是通过在股骨髁部植入一种新型的双网络凝胶，以明确是否可以在股骨髁形成的大范围骨软骨缺损中诱导自发的透明软骨再生。本研究选用成年兔25只。在每只动物的双侧膝关节中，我们在内侧髁突造成直径为2.4 mm的骨软骨缺损。然后，我们在21只兔的右膝关节缺损处植入了一个DN凝胶塞，这样就留下了1.5 mm深(I组)、2.5 mm深(II组)或3.5 mm深(III组)的空隙。在左膝，我们没有对缺陷进行任何处理以获得对照数据。术后4周处死动物，进行大体和组织学评价。其余4只兔接受与II组相同的处理，4周后进行实时荧光定量聚合酶链式反应分析。II组的缺损处充填了足够体积的透明软骨组织，富含蛋白多糖和2型胶原。Wayne的大体外观和组织学评分显示，II组显著大于I组、III组和对照组(P<；0.012)。Ⅱ组2型胶原、聚集素和SOX9mRNAs的相对表达水平显著高于对照组(p<；0.023)。本研究表明，在股骨髁部形成的骨软骨缺损处，通过在缺损处植入DN凝胶塞，可以在体内诱导自发的透明软骨再生，从而故意在缺损处留下大约2 mm深的空隙。这一事实促使我们提出了一种无细胞培养的创新策略来修复股骨髁部的骨软骨损伤。
Functional repair of articular osteochondral defects remains a major challenge not only in the field of knee surgery but also in tissue regeneration medicine. The purpose is to clarify whether the spontaneous hyaline cartilage regeneration can be induced in a large osteochondral defect created in the femoral condyle by means of implanting a novel double-network (DN) gel at the bottom of the defect. Twenty-five mature rabbits were used in this study. In the bilateral knees of each animal, we created an osteochondral defect having a diameter of 2.4-mm in the medial condyle. Then, in 21 rabbits, we implanted a DN gel plug into a right knee defect so that a vacant space of 1.5-mm depth (in Group I), 2.5-mm depth (in Group II), or 3.5-mm depth (in Group III) was left. In the left knee, we did not apply any treatment to the defect to obtain the control data. All the rabbits were sacrificed at 4 weeks, and the gross and histological evaluations were performed. The remaining 4 rabbits underwent the same treatment as used in Group II, and real-time PCR analysis was performed at 4 weeks. The defect in Group II was filled with a sufficient volume of the hyaline cartilage tissue rich in proteoglycan and type-2 collagen. The Wayne's gross appearance and histology scores showed that Group II was significantly greater than Group I, III, and Control (p < 0.012). The relative expression level of type-2 collagen, aggrecan, and SOX9 mRNAs was significantly greater in Group II than in the control group (p < 0.023). This study demonstrated that spontaneous hyaline cartilage regeneration can be induced in vivo in an osteochondral defect created in the femoral condyle by means of implanting the DN gel plug at the bottom of the defect so that an approximately 2-mm deep vacant space was intentionally left in the defect. This fact has prompted us to propose an innovative strategy without cell culture to repair osteochondral lesions in the femoral condyle.
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