Expression of Barhl2 and its relationship with Pax6 expression in the forebrain of the mouse embryo.
Expression of Barhl2 and its relationship with Pax6 expression in the forebrain of the mouse embryo.
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DOI：
10.1186/s12868-016-0311-6
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发表时间：
2016-11-25
期刊：
影响因子：
2.4
通讯作者：
Price DJ
中科院分区：
文献类型：
--
作者：
Parish EV;Mason JO;Price DJ
转录因子Barhl2是一种在神经元分化中起作用的抗原神经转录因子。其同源物在果蝇发育中的功能比其在哺乳动物大脑发育中的功能更容易理解。现有的证据表明，它在小鼠胚胎前脑中的表达是区域性的，并可能补充另一种对前脑发育很重要的转录因子Pax6。本研究的目的是提供一个更详细的描述Barhl2在胚胎前脑的表达模式比目前可用的，与Pax6的表达域，并检查Pax6损失对Barhl2表达的影响。我们发现，Barhl2表达在发育中的间脑从前神经管关闭的时间。它的表达最初重叠的Pax6在中央区域的间脑，但在接下来的日子里，他们的表达领域成为互补的前脑区域。例外的是丘脑和前顶盖，其中Pax6和Barhl2表达的反向梯度在胚胎第12.5天建立，在这些区域中的总体Pax6水平大幅下降而Barhl2水平保持相对较高之前。我们发现，Barhl2表达上调，特别是在丘脑和pretectum在Pax6基因敲除小鼠。Barhl2的区域特异性表达模式使其可能在胚胎小鼠前脑区域特异性差异的发展中发挥重要作用。抑制其在丘脑和顶盖前区的表达Pax6可能是至关重要的，允许proneural因子，以促进正常的神经元分化在这一地区。
The transcription factor Barhl2 is an antiproneural transcription factor with roles in neuronal differentiation. The functions of its homologue in Drosophila development are better understood than its functions in mammalian brain development. Existing evidence suggests that its expression in the embryonic forebrain of the mouse is regional and may complement that of another transcription factor that is important for forebrain development, Pax6. The aim of this study is to provide a more detailed description of the Barhl2 expression pattern in the embryonic forebrain than is currently available, to relate its expression domains to those of Pax6 and to examine the effects of Pax6 loss on Barhl2 expression. We found that Barhl2 is expressed in the developing diencephalon from the time of anterior neural tube closure. Its expression initially overlaps that of Pax6 in a central region of the alar diencephalon but over the following days their domains of expression become complementary in most forebrain regions. The exceptions are the thalamus and pretectum, where countergradients of Pax6 and Barhl2 expression are established by embryonic day 12.5, before overall Pax6 levels in these regions decline greatly while Barhl2 levels remain relatively high. We found that Barhl2 expression becomes upregulated in specifically the thalamus and pretectum in Pax6-null mice. The region-specific expression pattern of Barhl2 makes it likely to be an important player in the development of region-specific differences in embryonic mouse forebrain. Repression of its expression in the thalamus and pretectum by Pax6 may be crucial for allowing proneural factors to promote normal neuronal differentiation in this region.
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