Comparative ribosome profiling reveals distinct translational landscapes of salt-sensitive and -tolerant rice.
Comparative ribosome profiling reveals distinct translational landscapes of salt-sensitive and -tolerant rice.
复制标题
比较核糖体分析揭示了盐敏感和耐盐水稻的独特翻译景观
DOI：
10.1186/s12864-021-07922-6
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发表时间：
2021-08-12
期刊：
影响因子：
4.4
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Yang X;Song B;Cui J;Wang L;Wang S;Luo L;Gao L;Mo B;Yu Y;Liu L
背景
Background
Soil salinization represents a serious threat to global rice production. Although significant research has been conducted to understand salt stress at the genomic, transcriptomic and proteomic levels, few studies have focused on the translatomic responses to this stress. Recent studies have suggested that transcriptional and translational responses to salt stress can often operate independently.
Results
We sequenced RNA and ribosome-protected fragments (RPFs) from the salt-sensitive rice (O. sativa L.) cultivar ‘Nipponbare’ (NB) and the salt-tolerant cultivar ‘Sea Rice 86’ (SR86) under normal and salt stress conditions. A large discordance between salt-induced transcriptomic and translatomic alterations was found in both cultivars, with more translationally regulated genes being observed in SR86 in comparison to NB. A biased ribosome occupancy, wherein RPF depth gradually increased from the 5′ ends to the 3′ ends of coding regions, was revealed in NB and SR86. This pattern was strengthened by salt stress, particularly in SR86. On the contrary, the strength of ribosome stalling was accelerated in salt-stressed NB but decreased in SR86.
Conclusions
This study revealed that translational reprogramming represents an important layer of salt stress responses in rice, and the salt-tolerant cultivar SR86 adopts a more flexible translationally adaptive strategy to cope with salt stress compared to the salt susceptible cultivar NB. The differences in translational dynamics between NB and SR86 may derive from their differing levels of ribosome stalling under salt stress.
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影响因子：
7.2
作者：
Dever TE;Dinman JD;Green R
通讯作者：
Green R
DOI：
10.1126/science.1240585
发表时间：
2013-11-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fernández IS;Bai XC;Hussain T;Kelley AC;Lorsch JR;Ramakrishnan V;Scheres SHW
通讯作者：
Scheres SHW
DOI：
10.1038/nrm4069
发表时间：
2015-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Brar GA;Weissman JS
通讯作者：
Weissman JS
影响因子：
16
作者：
Huter, Paul;Arenz, Stefan;Wilson, Daniel N.
通讯作者：
Wilson, Daniel N.
影响因子：
1.5
作者：
Kumar, A.;Dwivedi, S.;Tripathi, R. D.
通讯作者：
Tripathi, R. D.